P.K. Arakelyan1, G.V. Raznicina1, N.F. Gaust1, S.K. Dimov2, K.S. Dimov2
1VNIIBTG RAAS, Russia, Omsk, st. Lermontova, 93
2IEVSSaFE RAAS, Russia, Novosibirsk region, Krasnoobsk, P.O. box 8
Summary. There were studied 4 schemes hyperimmunization healthy bulls, pre- tested for brucellosis with negative results, which hyperimmune (3 each head in each group): the alive culture of Brucella strain 19 n/a in doses of 75, 120 and 150 billion microbial cells with an interval of 10 days; alive culture of Brucella strain 19 n/a in doses of 50, 100 and 100 billion microbial cells with an interval of 10 days; killed Brucella culture strain19 n/a 50 billion microbial cells once combined with an adjuvant (5 ml); killed culture of Brucella strain 19 n/a 100 billion microbial cells once combined with an adjuvant (5 ml). The most effective was the scheme of the S-brucellosis diagnostic serum based on preliminary single subcutaneous hyperimmunization animals-producing killed culture vaccine strain of B. abortus 19 at a dose of 100 billion microbial cells in combination with the adjuvant MONTANIDE ISA 61 VG (5 ml), followed by two blood samples of 30 days after hyperimmunization. It is almost as good as the well-known scheme, which provides three-dose live vaccine strain of Brucella culture in achieving high diagnostic titers of the drug (average titers of RA and CFT after 6 months of storage 1166,7 and 1:80, respectively), but has the advantages associated with the epidemic safety as well as the possibility of obtaining (by adjuvant allowing to foster the production of high titers of antibodies even at single hyperimmunization) for the same period in twice the volume of serum.
Keywords: brucellosis, serological and bacteriological diagnosis, S-brucellosis diagnostic serum, methods of preparation, animals-producers, schemes hyperimmunization, antigens, Brucella species, antigenic relationship, virulent and vaccine strains, adjuvants, titers of diagnostic sera.