M.M. Kopus, N.G. Ignatieva, D.P. Dorokhova, N.S. Kravchenko, N.I. Sarycheva
All-Russian Research Institute of Grain Crops after I.G. Kalinenko, Nauchny Gorodok, 3, Zernograd, Rostovskaya obl., 347740, Russian Federation
Summary. In 2012-2014 in I.G. Kalinenko All-Russian Research Institute of Grain Crops the investigations were carried out. The aim of them was to identify varieties according to their alleles of prolamine proteins; to compile reference spectrums of the varieties passed to SVT; to determine biotype content and correlation of biotypes in polymorphic varieties; to find admixtures for further monitoring and maintaining of varietal (genetic) quality of seeds and their preservation (authenticity) in the primary seed-growing by means of laboratory methods. The material for the researches were eight promising varieties of soft winter wheat of breeding of the All-Russian Research Institute of Grain Crops: the varieties of the intensive type (Kipchak, Bonus, Kazachka, Luchezar) and varieties of the semi-intensive type (Kapitan, Kaprizulya, Admiral, Krasa Dona), which are being tested in the SVT at present. The electrophoresis of wheat proteins (gliadins) was carried out on a starch gel in aluminum lactate buffer (pH 3.1), identification of alleles of gliadin-coding loci was done according to the catalogue and SDS-sedimentation was carried out according to recommendations of the All-Russian Research Institute of Grain Crops. The Kaprizulya variety consists of two biotypes, the other seven studied varieties are monomorphous. It was identified admixture in the Kazachka variety (3%) and Admiral (2%). It was detected mutations in the Bonus variety – Gld 1D2 (a decrease in the intensity of the first component of the block) and Kazachka – Gld 1D0 (the deletion of the whole allele (block)). The varieties Luchezar and Krasa Dona showed similarity in gliadin spectrums.
Keywords: varieties, wheat, gliadins, polymorphism, biotype content, admixtures, variety seed quality.
Author Details: M.M. Kopus, D. Sc. (Biol.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N.G. Ignatieva, head of laboratory; D.P. Dorokhova, junior research fellow; N.S. Kravchenko, research fellow; N.I. Sarycheva, senior research fellow.
For citation: Kopus M.M., Ignatieva N.G., Dorokhova D.P., Kravchenko N.S., Sarycheva N.I. Biotype Composition and Identity of Varieties of Winter Wheat according to Grain Prolamines. Dostizheniya nauki i tekhniki APK. 2015. Vol. 29. No. 11. Pp. 52-54 (In Russ.)