FACTORS AFFECTING THE RESPONSE OF OVARIES OF DONOR COWS TO EXOGENOUS GONADOTROPIN (REVIEW)

A.V. Brigida¹, S. A. Bursakov¹, O. A. Skachkova¹, V. I. Sorokin², S. N. Kovalchuk^1
1Center for Experimental Embryology and Reproductive Biotechnologies, ul. Kostyakova, 12, str. 4, Moskva, 127422, Russian Federation
²Orenburg State Agrarian University, ul. Chelyuskintsev, 18, Orenburg, 460014, Russian Federation

Abstract. Embryo transfer is a widely used biotechnological method for accelerated reproduction of cattle. It is directed to the obtaining the maximum polyovulatory response of gametogenic organs to exogenous gonadotropins, and, accordingly, to the obtaining the maximum amount of high-quality embryos suitable for the transfer. However, the problem of the variability of the response of cows’ ovaries to gonadotropin FSH/LH preparations, which are generally recognized as the most effective, has not overcome. About 30% of animals do not show a response to exogenous gonadotropins, leading to the end of the embryo transfer procedure and economic losses. The quality of the ovarian response to induction of polyovulation has a multifactorial dependence, which makes it difficult to predict the effectiveness of this method and affects the economic expediency of the procedure. The aim of this review is to systematize the literature data on factors affecting the effectiveness of the response of ovaries of donor cows to the induction of polyovulation by exogenous gonadotropins. Special attention is paid to the search for prognostic criteria based on the development of molecular genetic markers (association of genes polymorphism with signs of polyovulatory response) that have a predictive ability. It is assumed that their use will make it possible to select donor cows with the highest type of ovarian response (25 and more dominant follicles per one stimulation session) and with 92–96% yield of high-quality oocytes that are capable of fertilization. It was reported about genes of the follicle-stimulating hormone receptor (FSH receptor), inhibin alpha (INHA), progesterone (PGR), growth factor and differentiation 9 (GDF9), luteinizing hormone/choriogonadotropin receptor (LHCGR), progesterone receptor, GnRHRI, and ionotropic glutamate receptor for alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid 1 (AMPA1/GRIA1) as predictors of superovulation. The predictive markers can be used to solve the problems of technological forecasting in the practice of production of bovine embryos and optimization of the technology of embryo transfer.

Keywords: cattle; embryo transfer; superovulation; reproduction; biomarkers.

Author Details: A. V. Brigida, head of division (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); S. A. Bursakov, Cand. Sc. (Biol.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); O. A. Skachkova, head of division (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); V. I. Sorokin, Cand. Sc. (Biol.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); S. N. Kovalchuk, Cand. Sc. (Biol.), acting director (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).

For citation: Brigida A.V., Bursakov S. A., Skachkova O. A., Sorokin V. I., Kovalchuk S. N. Factors Affecting the Response of Ovaries of Donor Cows to Exogenous Gonadotropin (Review). Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 6. Pp. 56-63 (in Russ.). DOI: 10.24411/0235-2451-2018-10614.