Relationship between PIT-1 (POU1F1) Gene Polymorphism with Milk Productivity and Reproductive Capacity of Holstein Cattle

E. R. Gaynutdinova¹, N. Yu. Safina^1,2, F. F. Zinnatova¹, Sh. K. Shakirov^1
1Tatarian Agricultural Research Institute – autonomous structural subdivision of the Federal Research Center of the Kazan Scientific Center, RAS, ul. Orenburgskii trakt, 48, Kazan’, 420059, Russian Federation
²N. E. Bauman Kazan State Academy of Veterinary Medicine, ul. Sibirskiy trakt, 35, Kazan’, 420029, Russian Federation

Abstract. The purpose of the research was to study the polymorphism of the PIT-1 gene with the replacement of G with A in the sixth exon region, as well as to evaluate the milk productivity and reproductive ability of Holstein heifers with different genotypes on this gene. The work was performed in 2016–2018 in the Republic of Tatarstan on 333 first-calf cows. Genotyping was conducted by PCR-RFLP in Hinf I locus of PIT-1 gene. The studied population was polymorphic, the genetic balance was not disturbed. The distribution of alleles and genotypes was as follows: A – 0.32 and B – 0.68; AA – 11.7% (39 cows), AB – 40.5% (135 cows), BB – 47.7% (159 cows). Animals with AA genotype of PIT-1 gene were not significantly better in terms of milk yield per 305 days of lactation (7339.6 kg), the mass fraction of protein (3.39%), milking capacity index (1367.8), and lactation stability coefficient (98.6%). The cows from the same group were statistically significantly superior to heifers with AB and BB genotypes in terms of productivity including the mass fraction of fat (4.05%), milk fat yield (297.2 kg), and milk protein output (248.8 kg). The animals with PIT-1 gene of AB genotype, which showed a low level of milk yield, a low content of fat mass fraction, a relatively low lactation stability coefficient and milk production index, were distinguished by the early age of the first successful insemination (17.3 months) and the shortest calving interval (376.6 days). First-calf cows of AB genotype were significantly better in terms of interlactation period (52.3 days) and service period (102.9 days), calf output (91.9 animals), reproduction rate (101.5%), and fertility index (57.7). The identified associations of PIT-1 gene polymorphism can be used for the selection of parental pairs for targeted breeding to improve economically useful signs of Holstein cattle.

Keywords: gene; allele; polymorphism; pituitary transcription factor; PIT-1 (POU1F1); cattle; productivity; milk yield; fat; protein; reproductive ability.

Author Details: E. R. Gaynutdinova, junior research fellow (Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N. Yu. Safina, post graduate student, research fellow; F. F. Zinnatova, Cand. Sc. (Biol.), deputy director; Sh. K. Shakirov, D. Sc. (Agr.), chief research fellow.

For citation: Gaynutdinova E. R., Safina N. Yu., Zinnatova F. F., Shakirov Sh. K. Relationship between PIT-1 (POU1F1) Gene Polymorphism with Milk Productivity and Reproductive Capacity of Holstein Cattle. Dostizheniya nauki i tekhniki APK. 2019. Vol. 33. No. 11. Pp. 69–73 (in Russ.). DOI: 10.24411/0235-2451-2019-11115.