O. I. Molkanova, O. V. Koroleva, I. L. Krakhmaleva
Tsytsyn Main Botanic Garden, Russian Academy of Sciences, ul. Botanicheskaya, 4, Moskva, 127276, Russian Federation
Abstract. The purpose of the study was to improve the methods of in vitro reproduction and preservation of representatives of the genus Syringa to maintain the collection of this culture. The work on the formation of the in vitro collection fund began in the 2000s. In vitro collection of the genus Syringa L. of Tsytsin Main Moscow Botanical Garden includes 238 varieties and selected forms, most of which (64%) were bred in the countries of the former USSR (Russia, Belarus, Ukraine, Kazakhstan). At the stage of actual propagation, modified Murashige and Skoog (MS), Quorin and Lepoivre (QL) media were used. They were added sucrose at a concentration of 30 g/L, growth regulators 6-BAP (6-Benzylaminopurine) at a concentration of 0.5–1.0 mg/L, and IAA (Indole-3-acetic acid) at a concentration of 0.01 mg/L. The control was MS culture medium without hormones. At the stage of deposition under conditions of slow growth, a modified Murashige and Skoog media were added sucrose at a concentration of 40 g/L and the retardant Chlorocholine Chloride (CCC) at a concentration of 0.2–0.4 mg/L. The control was MS medium with 40 g/L of sucrose without retardant. The modified Quorin and Lepoivre media were best suited for the cultivation of the representatives of the genus Syringa. The multiplication factor on it was 23% higher than on the Murashige and Skoog media. The combined inclusion of 6-BAP and IAA in the nutrient medium increased the multiplication factor of plants by 9%, regardless of the genotype and mineral base. The introduction of 1.0 mg/L of 6-BAP and 0.01 mg/L of IAA into the culture medium is recommended for effective propagation of most varieties. Adding 0.2–0.4 mg/L of Chlorocholine Chloride to the nutrient medium at the stage of preservation significantly reduced the growth processes of lilac microclones without loss of viability (the length of internodes reduced to 1–3 mm) that increased the period of plant subculturing.
Keywords: Syringa; in vitro collection fund; clonal micropropagation; culture media; growth regulators; long-term preservation.
Author Details: O. I. Molkanova, Cand. Sc. (Agr.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); O. V. Koroleva, junior research fellow; I. L. Krakhmaleva, junior research fellow.
For citation: Molkanova OI, Koroleva OV, Krakhmaleva IL. [The concept of forming a collection of representatives of the genus Syringa L. in in vitro culture]. Dostizheniya nauki i tekhniki APK. 2020;34(9):30-4. Russian. doi: 10.24411/0235-2451-2020-10906.