V. S. Vlasenko, E. A. Vishnevskii, T. S. Dudoladova
Omsk Agrarian Scientific Center, ul. Koroleva, 26, Omsk, 644027, Russian Federation
Abstract. The purpose of the research was to study the dynamics of the cytochemical activity of myeloperoxidase (MPO) and the content of lysosomal cationic proteins (LCP) in neutrophilic granulocytes of animals infected with leukemia at different periods of postnatal development. The work was performed at a farm with a high prevalence of bovine leukemia virus (BLV) in a herd of 80 animals of different ages: up to 2 months (n = 20), 4–6 months (n = 20), 9–12 months (n = 20), and 15–17 months (n = 20). According to the results of diagnostic studies using the polymerase chain reaction (PCR) method, animals of all age categories were divided into 2 groups: intact (control) and positively responding to PCR (infected with BLV). We found significant changes in the activity of antimicrobial systems of neutrophils in BLV carriers, compared with intact animals, in all observed age periods. In infected young cattle aged from 9 to 12 months, we revealed a unidirectional transformation, characterized by a statistically significant decrease in the number of inactive cells by 28.5–45.0%, an increase in the number of neutrophils with a high degree of activity of antimicrobial peptides by 16.4–41.5%, as well as an increase in their total value, expressed as an average cytochemical coefficient (ACC) by 14.3–36.8%. Young cattle aged from 15 months to 17 months were observed an opposite trajectory of changes, indicating the insufficiency of the enzymatic and non-enzymatic mechanisms of antimicrobial protection and manifested, ultimately, by a decrease in the ACC of LCP and MPO by 29.8% and 18.1%, respectively. The research results suggest the possibility of using cytochemical parameters of blood neutrophils to assess the predisposition of animals to infection with BLV at a dysfunctional farm.
Keywords: young cattle; leukemia virus; PCR; neutrophilic granulocytes; bactericidal systems; myeloperoxidase; cationic proteins.
Author Details: V. S. Vlasenko, D. Sc. (Biol.), chief research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E. A. Vishnevskii, post graduate student; T. S. Dudoladova, Cand. Sc. (Biol.), senior research fellow.
For citation: Vlasenko VS, Vishnevskii EA, Dudoladova TS. [Cationic proteins of lysosomes and myeloperoxidase in neutrophils of young cattle of different ages infected with leukemia] Dostizheniya nauki i tekhniki APK. 2021;35(5):65-9. Russian. doi: 10.24411/0235-2451-2021-10511.