Antagonistic bacteria of pathogens of intestinal infections and producers of a complex of cellulases as a basis for creating additives that combine the functions of a probiotic and a feed enzyme

L. R. Valiullin^1,2, Rish. S. Mukhammadiev^1,2, Rin. S. Mukhammadiev^1,2, V. I. Egorov², V. Yu. Rud², A. P. Glinushkin^2
1Federal Center of Toxicological, Radiation and Biological Safety, Nauchnyi gorodok, 3, Kazan, 420008, Russian Federation
²All-Russian Research Institute of Phytopathology, ul. Institut, vl. 5, r.p. Bol’shie Vyazemy, Odintsovskii r-n, Moskovskaya obl., 143050, Russian Federation

Abstract. The investigation evaluated the ability of microorganisms with antimicrobial action to synthesize extracellular cellulases to determine the possibility of creating additives that combine the functions of a probiotic and a feed enzyme. In total, 274 bacterial strains of various genera and species were studied. Their antagonistic and cellulase activity was determined by the plate method. Relatively high bactericidal activity against test cultures of Klebsiella pneumoniae, Salmonella typhimurium, Escherichia coli and Staphylococcus aureus were characteristic of 2.9%, 3.7%, 4.7% and 6.9% of the strains studied, respectively. Only six of 274 strains (2.2%) demonstrated a sufficiently high level of fungicidal activity against Aspergillus niger isolate and only five strains (1.8%) demonstrated a sufficiently high level of fungicidal activity against Fusarium moniliforme. The S-2 and LR86 Bacillus subtilis strains were characterized by the highest antimicrobial activity and the production of cellulolytic enzymes. They are able to secrete extracellular cellulases, which hydrolyze soluble and insoluble cellulose-containing substrates. The most intensive formation and release of carboxymethyl cellulase and avicelase into the culture medium during the cultivation of the B. subtilis LR86 strain occurred on the third day, while the enzymatic activity was (0.93 ± 0.02) units/mL and (0.26 ± 0.007) units/mL, respectively. Compared to the B. subtilis LR86 strain, the B. subtilis S-2 strain was characterized by a lower level of carboxymethylcellulase and beta-glucosidase production. Their activity was 1.9 and 1.2 times lower, respectively, and reached the highest values on the fifth day. The biosynthesis of B. subtilis S-2 exoglucanase and B. subtilis LR86 beta-glucosidase reached maximum values on the fourth day of growth; the enzyme activity was (0.17 ± 0.004) units/mL and (0.088 ± 0.002) units/mL, respectively.

Keywords: Bacillus subtilis; antimicrobial activity; cellulase complex; probiotics; feed enzymes; animal husbandry.

Author Details: L. R. Valiullin, Cand. Sc. (Biol.), leading research fellow (е-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); Rish. S. Mukhammadiev, Cand. Sc. (Biol.), research fellow; Rin. S. Mukhammadiev, Cand. Sc. (Biol.), research fellow; V. I. Egorov, Cand. Sc. (Biol.), research fellow; V. Yu. Rud’, D. Sc. (Phis.-Math.), leading research fellow; A. P. Glinushkin, D. Sc. (Agr.), Corresponding member of the RAS, director.

For citation: Valiullin L.R., Mukhammadiev RishS, Mukhammadiev RinS, et al. [Antagonistic bacteria of pathogens of intestinal infections and producers of a complex of cellulases as a basis for creating additives that combine the functions of a probiotic and a feed enzyme] Dostizheniya nauki i tekhniki APK. 2021;35(9):60-6. Russian. doi: 10.53859/02352451_2021_35_9_60.