Phenotypic variability of enzyme activity in full-aged ewes of the Romanov breed in Kuzbass

I. N. Morozov¹, O. I. Sebezhko², E. I. Tarasenko², E. A. Klimanova^2
1Kuzbass State Agricultural Academy, ul. Markovtseva, 5, Kemerovo, 650056, Russian Federation
²Novosibirsk State Agrarian University, ul. Dobrolyubova, 160, Novosibirsk, 630039, Russian Federation

Abstract. The study of blood serum enzymes activity allows to establish the objective state of the functional processes in the body in normal and pathological conditions. The studies aimed to determine the phenotypic variability of the activity of alkaline phosphatase (AP), lactate dehydrogenase (LDH), and creatine kinase (CK) in the blood of full-aged Romanov ewes in Kuzbass to assess the environmental and climatic trends in the adaptive potential of animals. Enzyme activity was measured by the kinetic method using the Alkaline Phosphatase-Novo, LDH-UV-Novo, and Creatine Kinase-Novo reagent kits manufactured by Vetor-Best (Russia) on a Photometer 5010 V5+ semi-automatic analyser. The content of LDH was characterised by a normal Gaussian distribution, in contrast to alkaline phosphatase and creatine kinase. The average content of alkaline phosphatase (313.6 u/l) and creatine kinase (98.6 u/l) in the blood serum of full-aged ewes of the Romanov breed was within the generally accepted physiological norm, the level of lactate dehydrogenase (1553±101 u/l) was increased. When calculating the correlation ratios, very weak positive and negative relationships between the levels of enzymes were revealed, which had no practical significance. The reference interval of alkaline phosphatase for full-aged ewes of the Romanov breed, bred under the climatic and geographical conditions of Kuzbass, was 70.83-604.2 pcs/l (with the lower limit of the 90% confidence limit of 6.02-7.8 pcs/l, upper – 520.1-689.9 pcs/l), lactate dehydrogenase – 288-2711.4 pcs/l (with confidence limits of 262-508.2 and 2435.2-3118 pcs/l, respectively), creatine kinase – 44.4-206.2 pcs/l (22.8-46.7 and 173.6-255.7 pcs/l).

Keywords: alkaline phosphatase; lactate dehydrogenase; creatine kinase; sheep; Romanov breed; enzymes.

Author Details: I. N. Morozov, post graduate student (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); O. I. Sebezhko, Cand. Sc. (Biol.), assoc. prof.; E. I. Tarasenko, post graduate student; E. A. Klimanova, post graduate student.

For citation: Morozov IN, Sebezhko OI, Tarasenko EI, et al. [Phenotypic variability of enzyme activity in full-aged ewes of the Romanov breed in Kuzbass] Dostizheniya nauki i tekhniki APK. 2022;36(6):61-5. Russian. doi: 10.53859/02352451_2022_36_6_61.