I.K. Apolinarieva, S.O. Baturin
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Summary. The research on enzymatic maceration efficiency for extraction of the full seeds from freshly harvested everbearing garden strawberry fruits was carried out. For maceration process, berry pulp was treated with enzymatic agents Cellolux F and Macerobacellin by “SibFarm Ltd.” industrial group production. Optimal conditions for enzyme activity on berry pulp were revealed: Cellolux F and Macerobacellin should be added in a successive manner with the precede pH adjustment up to 4,7 and 7 for each enzyme compound respectively. It was found that enzymatic treatment facilitates seed extraction process due to fracturing robust vascular bundles and pulp. Total dry matter content in 0.5 kg of fresh berries differed significantly compare to the control (3.39 – 4.00 g) and reached 0.29 – 0.87 g. It was shown that enzyme treatment substantially increases the percentage of seeds in the lower fraction of pulp-water mixture: when enzymes were added simultaneously the percentage of seeds was 99,7 % from total dry weight, when enzymes were added in a successive manner this parameter reached 100%. Thus, successive application of enzyme agents purifies strawberry seed from pulp entirely. Thousand seed-weight of seeds, extracted by successive enzyme application, was significantly lower in the upper fraction of the pulp-water mixture (0.29 – 0.33 g) compare to the control (0.35 - 0.42 g). It allows to conclude that this method is suitable for sorting out the light and hollow seeds with lower germination capacity. Application of Cellolux F and Macerobacellin opens new prospects for mass seed production in the breeding of everbearing seed-reproducible garden strawberries.
Keywords: seeds, everbearing garden strawberry, berries, enzymatic maceration, enzyme preparations Cellolux F and Macerobacellin, selection.