E.N. Gonchar, A.V. Shcherbakov, K.G. Lopatko, L.N. Gonchar, V.K. Chebotar, S.M. Kalenska
Summary. This study was conducted to determine the effectiveness of biogenic metals nanoparticles as a part of biological products based on Bacillus subtilis and Mezorizobium cicer, as well as features of the formation of symbiotic nitrogen fixation and yield of chickpea (variety Roseanne) depending on the options of seed inoculation in terms Right-bank forest-steppe of Ukraine (typical chernozem with low humus content) and North-West region of Russia (surface-podzolic combined with peat- podzolic-gley soils). Work carried out by the Center for collective use State Research Institute for Agricultural Microbiology RAAS and the National University of Life and Environmental Sciences of Ukraine. The optimal concentration of nanoparticles (NPs), which increased the biological product titer of rhizosphere bacteria by 1.11 billion CFU / ml compared to the control. It was shown that the growth-promoting properties have bass, thus found that the maximum effect was observed when co-processing of seeds of silver NP and culture B. subtilis CH13 – 105%. The scheme included two field trials experience factor: Factor A – seed treatment (1. Control (water); 2. Mezorizobium cicer strain H -12; 3. strain M. cicer ST282 4. nanosolution of molybdenum (Mo); 5. Mezorizobium cicer H-12 + nanosolution Mo; 6 . M. cicer ST282 + nanosolution Mo), Factor B – Mo concentration (1. 1:10 2 . 1:100). Account area – 25 m2. It was shown that the level of production indolyl-3-acetic acid (IAA) by Mezorizobium cicer depending for Mo concentration and synthesis of auxins increased at 97.2 %. This combination was most effective in field experiments, and the differences were observed as the level of bacterial strains and at concentration of molybdenum. The best result was in the variant with pre-treatment of chickpea seeds by Mezorizobium cicer strain H-12 and NP Mo 1:100 dilution, which in combination provided a yield increase at – 6.4 (RF) and 8.0 (Ukraine) c/ha. Thus, the usage of metal NP, as a component of bacterial fertilizers, can increase the titer of the bacterial culture and improve agronomic properties of bacterial preparations.
Keywords: nanoparticles, microelements, biofertilizers.