O.M. Minaeva1, M.V. Apenysheva1, E.E. Akimova1,2, P.A. Blinova2, A.V. Kurovskiy1
1Tomsk State University, 36, Lenina Avenue, Tomsk, 634050, Russia
2Siberian Research Institute of Agriculture and Peat, Gagarina Str., 3, Tomsk, 634050, Russia
Summary. We investigated the effect of rhizosphere bacteria on the activity of polyphenol oxidase and peroxidases of wheat under phytopathogenic load as a mechanism of formation of plant resistance to pathogens under laboratory conditions. Three types of bacteria were used for inoculation of wheat seeds: Pseudomonas sp. В-6798, P. aureofaciens BS 1393 and P. fluorescens АР-33. Bacteria were inoculated in quantity 1x10E6 cell/seed. The culture of the fungus Fusarium oxysporum was used as the infection load in the experiments. The oxidase activity was assessed by the titration in the acetate buffer. In the absence of phytopathogenic load after the inoculation, the activity of polyphenol oxidase increased in aboveground parts of plants. The level 6 times higher than control values was registered in the variant with inoculation by Pseudomonas sp. B-6798 (р < 0.05). With the presence of F. oxysporum and without bacteria inoculation the activity of this enzyme increased in plant roots 2.5 times, in comparison with the variant without the phytopathogen. In roots of inoculated plants the activity of polyphenol oxidase was almost invariable compared with the similar ones in the ecosystems without the pathogen. The peroxidase activity without phytopathogenic load increased after seed inoculation. For Pseudomonas sp. B-6798 the increase in peroxidase activity in the root part of plants was observed (2.5 times), and for other examined strains this value increase in the leaves (2.0...2.4 times). In the presence of F. oxysporum the peroxidase activity increased in aboveground parts and reduced in roots of control plants. In the case of seed inoculation the value of this index in roots exceeded the control 1.6...2 times. On the basis of obtained data on the changes of the activity of the studied enzymes in inoculated plants in laboratory experiments it is possible to test plant protection agents against wheat root rot to choose the strains, which increase the activity of peroxidases in plant roots.
Keywords: induced resistance, rhizobacterial systemic resistance, fusarious, peroxidase, polyphenol oxidase, seed inoculation, phytopathogens.
Author Details: O.M. Minaeva, Cand. Sc. (Biol.), Assoc. Prof., M.V Apenysheva, Post graduate student, E.E. Akimova, Cand. Sc. (Biol.), Assoc. Prof., Senior Researcher, P.A. Blinova, Junior Researcher, A.V. Kurowski, Cand. Sc. (Biology), Assoc. Prof.
For citation: Minaeva O.M., Apenysheva M.V., Akimova E.E., Blinova P.A., Kurowski A.V. Influence of wheat seed inoculation on oxidase activity in plants under phytopathogenic load // Dostizheniya nauki i tekhniki APK. 2015. T. 29. № 6. pp. 53-56 (In Russ)