I.P. Novgorodova1, E.A. Gladyr1, V.I. Fisinin2, N.A. Zinovieva1
1All-Russian Research Institute of Animal Husbandry named after academy member L.K.Ernst, 60, Dubrovitsy, Podolskiy r-n, Moskowskaya obl., Russian Federation
2All-Russian Research and Technological Institute of Poultry Husbandry, ul. Ptizegradskaya, 10, Sergiev-Posad, Moskowskaya obl., Russian Federation
Summary. The comparative assessment of 14 chicken breeds of meat and egg direction of productivity (n = 420) with the use of different number of microsatellite loci was carried out to determine population (breed) belonging of chickens. We studied the efficacy of two test-systems, each of them included eight markers – GAL-1 (MCW0111, MCW0067, LEI0094, MCW0123, MCW0081, MCW0069, MCW0104 и MCW0183) and GAL-2 (MCW0295, ADL0112, MCW0037, MCW0034, ADL0268, MCW0222, MCW0014 и LEI0074). The studied groups of birds were represented by the following breeds: Leningradskaya Belaya (LEN_B), Leningradskaya Sittsevaya (LEN_S), Moskovskaya Belaya (MOS_B), Pantsirevskaya Belaya (PAN_B), Pantsirevskaya Chernaya (PAN_CH), Pervomajskaya (PERV), Poltavskaya Glinistaya (POLT_GL), Yurlovskaya Golosistaya (YURL_G), Kotlyarovskaya (KOTL), Kuchinskaya (KUCH), Rhode Island Red (ROD_A), Sussex (SUS), Plymouth Rock Polosaty (PLIM_P) and New Hampshire (NYU_G). Genetic typing using multilocus panels was performed on the genetic analyzer ABI 3130x1 (Applied Biosystems, USA). Statistical data processing was performed using the software GenAlEx (ver.6.4.1.). There were no significant differences in the mean number of alleles, number of informative alleles and other indexes per locus between two studied test systems. The mean number of alleles per locus (Na) for the whole populations using 8 and 16 loci was 6.64 ± 0.24 and 6.16 ± 0.17, respectively. The comparative analysis of two test-systems revealed their high specificity. The mean value of the probability of identity depending on the population was up to 96.0% using 16 markers, while the same index reached 89% using 8 markers. The population assignment using microsatellites of the GAL-1 and GAL-2 systems was 100% in some breeds. The use of the test system based on 16 loci was the most preferred for this purpose.
Keywords: microsatellite analysis, meat and egg breeds of chickens, alleles, polymorphism, heterozygosity.
Author Details: I.P. Novgorodova, Cand. Sc. (Biol.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E.A. Gladyr, Cand. Sc. (Biol.), head of laboratory; V.I. Fisinin, member of the RAS, director; N.A. Zinovieva, member of the RAS, director.
For citation: Novgorodova I.P., Gladyr E.A., Fisinin V.I., Zinovieva N.A. Identification of Chicken Breeds on the Basis of Microsatellite Analysis. Dostizheniya nauki i tekhniki APK. 2015. V.29. No11. Pp. 88-90 (In Russ.)