T.E. Deniskova1, L.A. Kaneva2, E.A. Gladyr1, M.I. Selionova3, N.A. Zinovieva1
1All-Russian Research Institute of Animal husbandry named after academy member L.K. Ernst, pos. Dubrovitsy, 60, Podol’skii r-n, Moskovskaya obl., 142132, Russian Federation
2Pechora Experimental Station named after A.V. Zhuravsky of Research Institute of Agriculture of Republic Komi, pos. Zhuravskii, Ust’-Tsilemskii r-n, Respublika Komi, 169480, Russian Federation
3All-Russian Research Institute of Sheep and Goat Breeding, pereulok Zootekhnicheskii, 15, Stavropol’, 355017, Russian Federation
Summary. Microsatellite markers provide information on the current genetic situation in populations of farming animals. The aim of our work was to study the allele pool, genetic diversity of Pechora sheep and to estimate its genetic relationships with other sheep breeds using eleven microsatellite markers. The research was performed in the Center for Biotechnology and Molecular Diagnostics of L.K. Ernst Institute of Animal Husbandry. Tissue samples of Pechora sheep (PECH, n = 50) were originated from nucleus herds of Pechora Experimental Station and L.A. Kaneva’s farm (Komi Republic, Russia). Comparison groups were represented by Romanov (ROM, n=59), Tuvan short fat-tailed (TUV, n=50), Tsigai (TSIG, n=40), Altay mountain (ALT, n= 30) and Kuibyshev sheep (KUI, n =15). Microsatellite analysis was carried out using ABI 3130xl genetic analyzer. Mean allele number and effective allele number per locus were 8.00 ± 0.79 and 4.58 ± 0.46, respectively, that was on 1.17 and 0.48 alleles less comparing to the mean values for the whole sample. Group PECH was characterized by moderate heterozygotes deficiency (9.2%). PECH sheep were genetically closest to KUI breed based on estimated Ney genetic distances and FST values: 0.106 and 0.026, respectively. The genetic clustering, performed for the most probable number of clusters k = 6 showed, that the mean value of membership in the own cluster was Q3/6 = 0.821 ± 0.029. The values of the above-mentioned parameter in ROM, ALT, TSIG, TUV and KUI were Q2/6 = 0.827 ± 0.037, Q6/6 = 0.763 ± 0.039, Q5/6 = 0.665 ± 0.041, Q4/6 = 0.491 ± 0.054 and Q1/6 = 0.452 ± 0.086, respectively. Analysis performed at the individual level showed, that 80% animals in PECH group were characterized by membership values in the own cluster more or equal to 75%. Thus, our research result indicated that Pechora sheep have their own unique allele pool and are genetically differentiated from the other sheep breeds of the same productive type. The data will be used in the breeding program for genetic improvement of Pechora sheep.
Keywords: Pechora sheep, microsatellites, allele pool, genetic diversity.
Author Details: T.E. Deniskova, Сand. Sc. (Biol.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); L.A. Kaneva, post-graduate student, acting director; E.A. Gladyr, Сand. Sc. (Biol.), head of laboratory; M.I. Selionova, D. Sc. (Biol.), prof. of the RAS, director; N.A. Zinovieva, D. Sc. (Biol.), member of the RAS, director.
For citation: Deniskova T.E., Kaneva L.A., Gladyr E.A., Selionova M.I., Zinovieva N.A. Genetic Characteristic of Pechora Sheep using Microsatellite Markers. Dostizheniya nauki i tekhniki APK. 2016. V. 30. No. 8. Pp. 75-78 (in Russ.).