MARKER-ASSISTED SELECTION AND EXAMPLES OF ITS APPLICATION IN WORLD POTATO GROWING

E.K. Khlestkina^1,2, V.K. Shumny^1,2, N.A. Kolchanov^1,2
1Federal Research Center Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences, prosp. Lavrent’eva, 10, Novosibirsk, 630090, Russian Federation
²Novosibirsk State University, ul. Pirogova, 2, Novosibirsk, 630090, Russian Federation

Summary. The goal of the article was to determine the role of marker-assisted selection (MAS) in modern plant breeding in our country and abroad by analysis of the literature data and to evaluate prospects of MAS application in potato growing. A brief history of MAS development and preceding findings underlying establishing of the MAS approach as well as the key differences between MAS and previous approaches, which determined intensive introduction of MAS in breeding practice, are described. The analysis of publication activity in peer-reviewed journals indexed in the Scopus database revealed leading countries and the place of Russia in the ranking of publications describing the implementation of DNA markers in plant research, including potato research. The leading countries in MAS-based potato research are the USA, the Netherlands, Great Britain and Germany. Russia occupies the 23rd place, that is higher than in the whole plant research ranking, but lower than in wheat ranking, in which Russia occupies the 12th place. MAS application for different breeding tasks in potato research was assessed. It is more often used for breeding for resistance to pathogens and pests (in this field MAS is in the greatest demand in the world), then for yield (the 2nd place) and abiotic stress resistance (the 3rd place). Advantages of marker-assisted selection such as economic efficiency, shortening time spent for breeding and possibility to create genotypes with unique combinations of genes are described and illustrated by examples. According to estimates given in the literature, costs for field assessment are 3 times higher than for DNA analysis in potato breeding for nematode resistance. The use of MAS allowed increasing the resistance to the pale potato cyst nematode almost an order of magnitude as a result of the pyramiding of two resistance genes. The article also outlined current directions for the development of new diagnostic DNA markers that are important for the development of the national potato breeding programs.

Keywords: potato breeding, potato genetics, disease resistance, diagnostic DNA-markers, gene pyramiding.

Author Details: E.K. Khlestkina, D.Sc. (Biol.), professor of the RAS, head of division¹; chief research fellow of research center² (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); V.K. Shumny, D.Sc. (Biol.), member of the RAS, adviser of the RAS1, prof.²; N.A. Kolchanov, D.Sc. (Biol.), member of the RAS, director¹, head of department².

For citation: Khlestkina E.K., Shumny V.K., Kolchanov N.A. Marker-Assisted Selection and Examples of Its Application in World Potato Growing. Dostizheniya nauki i tekhniki APK. 2016. V.30. No. 10. Pp. 5-8 (in Russ.).