PHYTOSTEROL CONTENT IN PULP AND SEEDS OF SEA-BUCKTHORN VARIETIES OF DIFFERENT ENVIRONMENTAL AND GEOGRAPHICAL ORIGIN

A.Y. Zemtsova¹, Y.A. Zubarev¹, A.V. Gunin¹, S. Kuehn^2
1Lisavenko Research Institute of Horticulture for Siberia, Zmeinogorskiy Tract, 49, Barnaul, 656045, Russian Federation
²UBF GmbH, An der Muhl, 1, Altlandsberg, 15345, Germany

Abstract. We studied the phytosterol content in pulp and seeds of 15 sea-buckthorn varieties belong to different environmental and geographical origin, growing under similar soil and climatic conditions (the forest-steppe of Altai Krai). The samples for biochemical analysis was collected in 2014-2015 on the plot for variety testing of the M.A. Lisavenko Research Institute of Horticulture for Siberia, located on the left high bank of the Ob, on the Ob plateau. Most of the samples belong to H. rhamnoides ssp. mongolica and include pure ecotype representatives, the forms, created by crossings of different ecotypes, the samples, developed by chemical mutagenesis; and four samples not included in mongolica ssp. were also studied. Phytosterol content was analyzed by gas chromatograph Shimadzu GC-14 A. Sterols were identified according to retention time during separation of the standard mixtures of following substances: beta-sitosterol, campesterol, stigmasterol, brassicasterol, ergosterol. Beta-sitosterol prevails in the composition of phytosterols, the other substances was determined in trace quantities. The concentration of beta-sitosterol in the pulp of sea-buckthorn berries averaged (30.65 ± 1.72) mg/100 g, in seeds – (26.67 ± 1.98) mg/100 g over two years of investigation. Among different ecotypes the samples, obtained by the chemical mutagenesis, were characterized by the high content of this substance in pulp (37.66 ± 7.66 mg/100 g); the least its quantity was registered in variety samples of the hybrid generation of Sayansky x Katunsky ecotype (25.75 ± 4.25 mg/100 g). Variability of beta-sitosterol content in sea buckthorn seeds of different ecotypes was 27.29 %. The maximum content of beta-sistosterol was noted in Buryatsky ecotype (41.18 ± 0.47 mg/100 g), the minimum one – in Dynajsky ecotype (15.04 ± 4.50 mg/100 g). It was not determined any direct and stable correlations in dynamics of accumulation of beta-sitosterol in sea-buckthorn berries depending on harvesting time. On the average over two years the maximum was noted at the second date of sampling in the pulp of the most varieties. In seeds the dynamics of the sign changes was multidirectional.

Keywords: sea-buckthorn, beta-sitosterol, varieties, ecotypes, pulp, seed, harvest period.

Author Details: A.Y. Zemtsova, junior research fellow (е-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); Y.A. Zubarev, Cand. Sc. (Agr.), head of laboratory; A.V. Gunin, Cand. Sc. (Agr.), senior research fellow; S. Kuehn, head of laboratory (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).

For citation: Zemtsova A.Y., Zubarev Y.A., Gunin A.V., Kuehn S. Phytosterol Content in Pulp and Seeds of Sea-buckthorn Varieties of Different Environmental and Geographical Origin. Dostizheniya nauki i tekhniki APK. 2017. V. 31. No 2. Pp. 35-38 (in Russ.).