P.A. Frantsuzov1, M.M. Nikitin1, A.M. Mal’ko2, A.B. Zhivykh2, N.V. Statsyuk3, V.G. Dzhavakhiya3, A.G. Golikov1
1OOO «GenBit», pr. Nauchnyi, 20, str. 2, Moskva, 117246, Russian Federation
2Russian Agricultural Center, Orlikov per., 1/11, str. 1, Moskva, 107139, Russian Federation
3All-Russian Research Institute of Phytopathology, ul. Institut, vl. 5, r.p. Bol’shie Vyazemy, Odintsovskii r-n, Moskovskaya obl., 143050, Russian Federation
Abstract. Information about the phytosanitary state of potato fields in Russia is rather limited and includes either data on single pathogens or detailed data for separate regions. In this study we evaluated a potential of PCR/RT-PCR micromatrices, intended for simultaneous detection of a range of viral, bacterial, and oomycetal potato pathogens, for a wide-scale screening of the target pathogens. The study was carried out in 2015–2017 at the regional branches of the Russian Agricultural Center (”Rosselkhoztsentr”) in Kaliningrad, Leningrad, Tver, Moscow, Kostroma, Nizhny Novgorod, Samara and Irkutsk regions, Stavropol and Krasnodar Krais, as well as in the Republic of Tatarstan using an “AriaDNA” microchip amplifier and compatible microarrays. Samples were collected according to standard methods. The level of infection with RNA-viruses, according to the analysis of potato leaves (n = 580), was 28.8%, with the prevalence of PVS, PVYo and PVM viruses (46.2, 45.7, and 34.7% of the total number of infected samples, respectively). The level of mixed viral infections was 12.9% (n = 75) of the total number of samples. In the most cases, such infections were represented by a combination of two different viruses (73.3% of the total number of infected samples), while the rest of samples were infected by three or four viruses (16.0 and 10.7%, respectively). The mixed infections were dominated by PVYo, PVM, and PVS. Among the tuber samples (n = 456), it was revealed 19.1% of tubers, infected with DNA-containing pathogens, mainly Phytophthora infestans, Clavibacter michiganensis subsp. sepedonicus, Pectobacterium carotovorum subsp. carotovorum, and P. atrosepticum (35.6, 28.9, 18.9, and 13.3% of the total number of infected samples, respectively). Mixed bacterial infections were detected in one sample from Moscow region (P. carotovorum subsp. carotovorum + P. atrosepticum) and two samples from Kostroma region (C. michiganensis subsp. sepedonicus + P. carotovorum subsp. carotovorum). The tested technology represents a simple and informative tool to control a phytosanitary situation in relation to the most important potato pathogens.
Keywords: real-time PCR, matrix format, potato viral diseases, potato bacterial diseases, screening, phytosanitary monitoring.
Author Details: P.A. Frantsuzov, Cand. Sc. (Biol.), research biochemist (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); M.M. Nikitin, Cand. Sc. (Biol.), leading research engineer; A.M. Mal’ko, D. Sc. (Agr.), director; A.V. Zhivykh, Cand. Sc. (Agr.), director of division; N.V. Statsyuk, Cand. Sc. (Biol.), senior research fellow; V.G. Dzhavakhiya, Cand. Sc. (Biol.), leading research fellow; A.G. Golikov, D. Sc. (Chem.), deputy director.
For citation: Frantsuzov P.A., Nikitin M.M., Mal’ko A.M., Zhivykh A.B., Statsyuk N.V., Dzhavakhiya V.G., Golikov A.G. Large-Scale Screening of RNA- and DNA-containing Potato Pathogens Using PCR in a Matrix Format. Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 3. Pp. 45-49 (in Russ.). DOI: 10.24411/0235-2451-2018-10309.