N. Yu. Safina1,2, F. F. Zinnatova1, Yu. R. Yulmeteva1, Sh. K. Shakirov1, T. M. Akhmetov2, E. R. Gaynutdinova1
1Tatar Scientific Research Institute of Agriculture – Subdivision of the Federal State Budgetary Institution of Science “Kazan Scientific Center of Russia Academy of Sciences”, ul. Orenburgskii trakt, 48, Kazan’, 420059, Russian Federation
2N. E. Bauman Kazan State Academy of Veterinary Medicine, ul. Sibirskiy trakt, 35, Kazan’, 420029, Russian Federation
Abstract. The aim of the work was to study the beta-lactoglobulin gene polymorphism in the Tatarstan population of Holstein breed, to establish the effect of different genotypes on milk productivity indicators and economically useful traits. The work was performed in 2017 in the Atninsky district of the Republic of Tatarstan on 211 first-calf cows. Genotyping by the PCR-RFLP method of Hae III locus of LGB gene showed that the studied population was polymorphic and the genetic equilibrium was not disturbed. The frequency of allele A was 0.47, of allele В – 0.53; of genotype АА – 20.8% (44 animals), АВ – 49.8% (105 animals), ВВ – 29.4% (62 animals). For the first lactation (305 days) the animals with genotype AA significantly exceeded animals with genotypes AB and BB in milk yield. The milk yield of the animals with genotype AA was 7189.8 kg, the increase was 269.5 and 105.6 kg, respectively. In terms of the mass fraction of fat, first-calf heifers with genotypes AB and BB showed the best result (3.91 and 3.86%), the smallest value of this indicator was found in individuals with genotype AA (3.59%). The first-calf cows with homozygous genotype AA showed a positive trend in the mass fraction of protein (3.37%) and the yield of milk protein (242.6 kg). A slight difference with genotype AB was 0.02% and 10.1 kg; with BB – 0.05% and 7.0 kg, respectively. First-calf heifers with LGB(BB) genotype had a positive correlation between milk yield and fat (r = 0.02), milk yield and protein content (r = 0.02), fat content and protein content (r = 0.06), and milk yield and live weight at 18 months (r = 0.20). The identified relationships and correlations indicate possible genetic improvements in the studied herd using marker-assisted breeding.
Keywords: gene; genotype; beta-lactoglobulin; polymorphism; productivity; milk yield; fat; protein; correlation; first-calf heifers.
Author Details: N. Yu. Safina, junior research fellow, post graduate student (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); F. F. Zinnatova, Cand. Sc. (Biol.), deputy director; Yu. R. Yulmeteva, Cand. Sc. (Biol.), senior research fellow; Sh. K. Shakirov, D. Sc. (Agr.), chief research fellow; T. M. Akhmetov, D. Sc. (Biol.), vice-rector; E. R. Gaynutdinova, junior research fellow.
For citation: Safina N. Yu., Zinnatova F. F., Yulmeteva Yu. R., Shakirov Sh. K., Akhmetov T. M., Gaynutdinova E. R. Polymorphism of Beta-Lactoglobulin Gene (LGB) and Its Association with Economically Important Traits of Holstein Cattle. Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 9. Pp. 78–80 (in Russ.). DOI: 10.24411/0235-2451-2018-10918.