DEVELOPMENT OF A MULTIPLEX STR PANEL FOR THE PARENTAGE CONTROL AND CHARACTERISTICS OF GENETIC DIVERSITY OF GOATS

S. N. Petrov¹, V. R. Kharzinova¹, E. A. Gladyr¹, C. S. Sambu-Khoo², N. A. Zinovieva^1
1L. K. Ernst All-Russian Research Institute of Animal Husbandry, pos. Dubrovitsy, 60, Podol’skii r-n, Moskovskaya obl., 142132, Russian Federation
²Tyva Agricultural Research Institute, ul. Bukhtueva, 4, Kyzyl, Respublika Tyva, 667005, Russian Federation

Abstract. Panels of microsatellite markers, which are also known as STRs (short tandem repeats), are widely used for the parentage control, as well as an assessment of the allele pool state and the genetic structure of breeds and populations of farm animals. However, the studies of Russian goat breeds using this type of DNA markers have not been carried out to date. The goal of this work was to develop a multiplex panel for goat microsatellites analysis and to evaluate its information capacity. This work was carried out in 2018. The studied sample set included three breeds of goats: Soviet Wool (n = 32, the Republic of Tuva), Saanen (n = 42, Leningrad region) and Alpine (n = 23, Ekaterinburg). The developed panel consisted of 8 STR-loci (INRA006, ILSTS087, CSRD247, OarFCB020 INRA023, MCM527, SRCRSP024, and ILSTS011) and the amelogenin locus for sex identification. Ear tissue samples were used as biological material. Polymorphism of nine markers, grouped into one panel, was determined by the authors’ protocol using ABI 3130xl DNA analyzer (USA). Statistical analysis was performed in MS Excel 2007 with GenAIEx v. 6.5 plugin. All microsatellite loci, included in the panel, were polymorphic. The number of alleles in the loci varied from 4 (OARFCB20) in Alpine goats to 11 (ILSTS087 and CSRD247) in Soviet Wool goats. The probability of matching genotypes of individuals (PI) was practically excluded; the probability of exclusion as parents if the genotypes of both were known (РХ1) was more than 99% for all breeds. Soviet Wool goats had the highest level of genetic diversity, estimated by the average number of alleles and the average number of effective alleles per locus and by Shannon index: 8.63 ± 0.63, 4.65 ± 0.42 and 1.73 ± 0.08, respectively. The obtained results confirmed the high information capacity of the developed STR panel, both for the parentage control and for the estimation of the biodiversity of goat breeds.

Keywords: microsatellites; parentage validity; genetic diversity; goats.

Author Details: S. N. Petrov, Cand. Sc. (Biol.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); V. R. Kharzinova, Cand. Sc. (Biol.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E. A. Gladyr, Cand. Sc. (Biol.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); C. S. Sambu-Khoo, Cand. Sc. (Agr.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N. A. Zinovieva, D. Sc. (Biol.), member of the RAS, director.

For citation: Petrov S. N., Kharzinova V. R., Gladyr E. A., Sambu-Khoo C. S., Zinovieva N. A. Development of a Multiplex STR Panel for the Parentage Control and Characteristics of Genetic Diversity of Goats. Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 11. Pp. 60–63 (in Russ.). DOI: 10.24411/0235-2451-2018-11116.