O. V. Kostyunina, L. V. Getmantseva, M. S. Fornara, Y. A. Prytkov, N. A. Volkova
Federal Scientific Center of Animal Husbandry – L. K. Ernst All-Russian Research Institute of Animal Husbandry, pos. Dubrovitsy, 60, Podol’skii r-n, Moskovskaya obl., 142132, Russian Federation
Abstract. PRKAG3 gene was considered as a gene, characterized the qualitative characteristics of meat. It plays a key role in the regulation of carbohydrate and lipid metabolism. The purpose of the research was to evaluate the effect of T30N, S52G and I199V mutations on PRKAG3 gene on the quality indicators of pork. The object of the experiments was three-breed young F2 hybrid (Large White x Landrace x Duroc) hogs-analogues (n = 25). The pigs were grown under conditions of the physiological yard and were slaughtered at the age of 203–208 days. The basic diet and husbandry were identical and corresponded to zoohygienic norms. DNA was isolated using the DNA-Extran kit. The polymerase chain reaction was conducted and genotypes were determined according to PRKAG3 gene under the previously developed method. Muscle tissue was sampled after slaughter. For statistical analysis of the results a linear model was used. The frequencies of T and N alleles in p.T30N position were 0.88 and 0.12 respectively. The frequencies of NT and TT genotypes were 24 and 76%, respectively. The frequencies of G and S alleles at p.S52G position were 0.90 and 0.10, respectively. The frequencies of GG and SG genotypes were 80.0 and 20.0%, respectively. The frequencies of alleles I and V in p.I199V position were 0.70 and 0.30, respectively. The frequencies of II, IV and VV genotype were 44.0, 48.0 and 8.0%, respectively. In the meat of heterozygous NT animals the content of adipose tissue was significantly higher (by 2.08%) (5.34 ± 0.47%), than in the meat of TT genotype carriers. The content of hydroxyproline and adipose tissue was significantly higher in the meat of heterozygous IV individuals (51.36 ± 1.30 mg/% and 4.91 ± 0.58%) than in the meat of the carriers of genotype II – by 4.19 mg/% and 2.32%, respectively. Genetic variants on PRKAG3 gene are associated with the qualitative characteristics of the meat of three-breed hybrids and can be used to improve consumer properties of pork.
Keywords: pigs; DNA marker; PRKAG3; hybrid; intramuscular fat; polymorphism.
Author Details: O. V. Kostyunina, D. Sc. (Biol.), head of laboratory, leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); L. V. Getmantseva, Cand. Sc. (Agr.), leading research fellow; M. S. Fornara, Cand. Sc. (Biol.), senior research fellow; Y. A. Prytkov, Cand. Sc. (Biol.), research fellow; N. A. Volkova, D. Sc. (Biol.), head of laboratory, chief research fellow, professor of the RAS.
For citation: Kostyunina O. V., Getmantseva L. V., Fornara M. S., Prytkov Y. A., Volkova N. A. Evaluation of the Polymorphism Effect in the PRKAG3 Gene on the Quality Indicators of Pork. Dostizheniya nauki i tekhniki APK. 2019. Vol. 33. No. 4. Pp. 67–69 (in Russ.). DOI: 10.24411/0235-2451-2019-10417.