M. K. Zinchenko
Upper Volga Federal Agrarian Scientific Center, ul. Tsentral’naya, 3, pos. Novyi, Suzdal’skii r-n, Vladimirskaya obl., 601261, Russian Federation
Abstract. To assess the dependence of the ecological state of grey forest soil on the tillage methods and fertilizer systems in various agrolandscapes, we conducted microbiological monitoring of the number and ratio of bacteria using various forms of nitrogen. The work was conducted under conditions of the Upper Volga in 2011–2018. Soil samples were taken in May, July, and September from a 0–20 cm soil layer. The studies were conducted against contrasting intensification backgrounds, the fertilizer doses for which were calculated by the balance method taking into account the natural soil fertility. For 8 years, we observed the trend of a decrease in the number of proteolytic (the medium was meat-and-peptone agar, MPA) and amylolytic (the medium was starch-and-ammonia agar, SAA) ecological and trophic groups of microorganisms against mineral intensification backgrounds. Their total number against an intense and highly intense mineral background was in the range of 7.5 million, which was statistically significantly (LSD05 = 1.1 million CFU/g) lower than against organomineral backgrounds. A similar pattern was noted in the distribution of aerobic diazotrophs (Ashby medium). After moldboard ploughing and flat-cut loosening, the number of diazotrophs varied from 2.2 to 2.9 million CFU/g of soil. When cultivating clover against all backgrounds of intensification, the number of these microorganisms increased, compared to their number under cereals, 1.9–2.8 times. We noted reduced transformation rates of organic matter against an intense and highly intense mineral backgrounds. This was due not only to a reduction in the pool of ammonifiers and immobilizers of mineral nitrogen but also to their ratio. The pool of aminoautotrophic microflora increased, which significantly activated the processes of microbiological immobilization of soil nitrogen, compared with the processes of mortmass transformation. The seasonal dynamics of the microorganisms of the group of immobilizers was more pronounced than in the amino heterotrophic microflora.
Keywords: biomonitoring; microbocenosis; number of microorganisms; intensification levels; grey forest soil.
Author Details: M. K. Zinchenko, Сand. Sc. (Biol.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Zinchenko M. K. Monitoring of Bacterial Microflora in Agroecosystems of Grey Forest Soil. Dostizheniya nauki i tekhniki APK. 2019. Vol. 33. No. 12. Pp. 10–14 (in Russ.). DOI: 10.24411/0235-2451-2019-11202.