M. Y. Peturenko, N. V. Bezler, A. S. Hussein
A. L. Mazlumov All-Russian Research Institute of Sugar Beet and Sugar, pos. VNIISS, 86, Ramonskii r-n, Voronezhskaya obl., 396030, Russian Federation
Abstract. The biological collection of effective and phytopathogenic microorganisms of the Central Chernozem Region of the Mazlumov All-Russian Research Institute of Sugar Beet and Sugar includes 13 strains of bacteria of the genus Pseudomonas, isolated from soil, rhizosphere and rhizoplans of sugar beet (Beta vulgaris L.). All strains have an amplicon of 618 bps, characteristic of Pseudomonas sp., while strains No. 36, 67, 116, and 117 belong to the species P. fluorescens. The purpose of the study was to screen the collection strains for the presence of syrB and hrpZ genes responsible for the production of toxins characteristic of the pathovar Pseudomonas syringae pv. aptata, as well as to determine the prospects for their use in the production of biological products for agriculture. Pseudomonas sp. No. 75, 77, 91/2, 110, and P. fluorescens 116 strains showed the ability to grow on Ashby’s nitrogen-free medium, produce indolyl-3-acetic acid and free amino acids; they can be used to produce preparations that increase sugar beet productivity. These strains possess arginine dihydrolase and oxidase activity, which is not typical for most representatives of the P. syringae species and their pathovars; they are not capable of producing syringomycin toxin. PCR analysis with B1/B2 primer specific for syrB gene showed the absence of an appropriate amplification product of 752 bps. That is, among the studied strains, there are no representatives of the phytopathogenic species P. syringae. PCR analysis with hrpZ-F/hrpZ-R primer to identify a gene encoding harpine proteins showed the absence of a product of 613 bp in the strains No. 75, 77, 91, 91/2, 97, 103, 110, 113, 115 that made it possible to exclude the presence of Pseudomonas syringae pv. aptata in the phytopathogenic pathovar collection causing leaf blight of sugar beet.
Keywords: Pseudomonas syringae pv. aptata; syringomycin; syrB; hrpZ; sugar beet; polymerase chain reaction (PCR).
Author Details: M. Y. Peturenko, Cand. Sc. (Agr.), junior research fellow (е-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N. V. Bezler, D. Sc. (Agr.), head of laboratory; A. S. Hussein, Cand. Sc. (Agr.), senior research fellow.
For citation: Peturenko MY, Bezler NV, Hussein AS. [Screening of Pseudomonas bacteria associated with Beta vulgaris L. for signs of pathogenicity and syrB and hrpZ genes encoding them]. Dostizheniya nauki i tekhniki APK. 2020;34(1): 16-9. Russian. doi: 10.24411/0235-2451-2020-10103.