N. P. Tarabukina1, M. P. Neustroev1,2, S. V. Dulova1, A. M. Stepanova1, M. P. Skryabina1
1Federal Research Center “Yakutsk Scientific Center”, Siberian branch of the RAS, M. G. Safronov Yakutsk Research Institute of Agriculture, ul. Bestuzheva-Marlinskogo, 23/1, Yakutsk, 677001, Russian Federation
2Yakutsk State Agricultural Academy, Sergelyakhskoe sh. 3 km, 3, Yakutsk, 677007, Russian Federation
Abstract. Biological preparations are an alternative to traditional disinfectants. The purpose of the study was to test a new sanitary and hygienic preparation with B. subtilis strains for treating the cows’ udders. We studied the experimental series of Probiodes 3 + 5 preparation containing 1% of NaCl, 0.5% of NaHCO3, TNP-3 and TNP-5 B. subtilis strains (at a concentration of 1.5 x 10E5 CFU/cm3 each); pH of the solution was 9.3 units. The studies were conducted in a livestock complex in the Republic of Sakha (Yakutia). For this purpose, two groups of milk cows were formed according to the analogue principle. The duration of the experiment was one month. In the experimental group, the cows’ udders was processed after milking with Probiodes 3 + 5 preparation by spraying 1 mL of the test substance onto each nipple after removing the milking machine. In the control group, the preparation was not used. We selected swabs from the surface of the nipples’ skin of the cows. The effectiveness of sanitization was determined by the level of bacterial contamination: the number of mesophilic aerobic and facultative anaerobic microorganisms (MAFAMs), staphylococci and enterobacteria. We used generally accepted methods of microbiological research. The number of MAFAMs in swabs from the nipples of the cows from the experimental group was 42.6 times lower than in the control group; the number of staphylococci was 4.4 times lower; the number of enterobacteria was 25.8 times lower. Over the 30-day period of application of Probiodes 3 + 5, the incidence of cows mastitis decreased by 5.7%. According to the results of the study, we received Russian Federation patent “Method for sanation of the udder using B. subtilis bacteria strains” No. 2694204 dated July 9, 2019.
Keywords: Bacillus subtilis; udder sanation; bacterial contamination; staphylococcus; enterobacteria; mastitis.
Author Details: N. P. Tarabukina, D. Sc. (Vet.), head of laboratory (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); M. P. Neustroev, D. Sc. (Vet.), head of laboratory; S. V. Dulova, post graduate student; A. M. Stepanova, Cand. Sc. (Vet.), senior research fellow; M. P. Skryabina, Cand. Sc. (Vet.), leading research fellow.
For citation: Tarabukina NP, Neustroev MP, Dulova SV, et al. [New sanitary and hygienic agent with Bacillus subtilis strains]. Dostizheniya nauki i tekhniki APK. 2020;34(1): 52-5. Russian. doi: 10.24411/0235-2451-2020-10110.