Use of commercial media designed for human embryos for in vitro cultivation of cattle gametes and embryos

I. G. Smetanina¹, L. V. Tatarinova¹, A. S. Krivokharchenko^2
1All-Russian Research Institute of Physiology, Biochemistry, and Nutrition of Animals, branch of the Federal Research Center for Animal Husbandry – Ernst All-Russian Research Institute for Animal Husbandry, Borovsk, pos. Institut, Kaluzhskaya obl., 249013, Russian Federation
²Semenov Institute of Chemical Physics, Russian Academy of Sciences, ul. Kosygina, 4, Moskva, 119991, Russian Federation

Abstract. A number of commercial media have been developed for the cultivation of human gametes and embryos. The purpose was to study the effectiveness of their use in in vitro experiments on cattle. In the work, we used commercial media Cook, Medicult, Sage, and LifeGlobal, as well as media developed in our laboratory (own-produced media). The following combinations were studied: IVF – medium for fertilization (own-produced) + IVC – medium for cultivation (own-produced); IVF (own-produced) + IVC (Cook); IVF (Cook) + IVC (Cook); IVF (own-produced) + IVC (Medicult); IVF (Medicult) + IVC (Medicult); IVF (Sage) + IVC (Sage); IVF (own-produced) + IVC (Life Global); IVF (Life Global) + IVC (Life Global). Medicult media were proved to be the least efficient. They were characterized by low cell-division (approximately 20%) and the lack of full development to advanced stages (single morulae when used a commercial medium for fertilization). In Sage media, embryos developed better – 7.7% of fertilized oocytes reached the morula/blastocyst stage. In the experiments with Cook media, one fundamental aspect was revealed – it is advisable to use a special, own-produced IVF medium. In this case, 2 times more fertilized oocytes (56.3%) entered into division than in the case with the commercial IVF medium (29.4%). The same can be said for LifeGlobal media that showed the best results. The own-produced IVF medium increased the initial cell-division 2 times (59.4% versus 28.1%). LifeGlobal medium provided the greatest development of embryos to preimplantation stages (24.8%).

Keywords: gametes; commercial media for human embryos; cattle; in vitro cultivation.

Author Details: I. G. Smetanina, Cand. Sc. (Biol.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.; L. V. Tatarinova, leading specialist; A. S. Krivokharchenko, Cand. Sc. (Biol.), research fellow.

For citation: Smetanina IG, Tatarinova LV, Krivokharchenko AS. [Use of commercial media designed for human embryos for in vitro cultivation of cattle gametes and embryos]. Dostizheniya nauki i tekhniki APK. 2020;34(2):53-6. Russian. doi: 10.24411/0235-2451-2020-10211.