T. E. Deniskova, N. А. Radjabov, V. A. Bagirov, N. A. Zinovieva
Federal Scientific Center of Animal Husbandry –Ernst All-Russian Research Institute of Animal Husbandry, pos. Dubrovitsy, 60, Podol’skii r-n, Moskovskaya obl., 142132, Russian Federation
Abstract. An interspecific hybridization is a promising approach to transforming the gene pool of farm animals by introducing valuable alleles of wild species. This work aimed to assess the allelic and genetic diversity of three hybrid forms of sheep in order to develop scientifically-based programs for their use in the sheep industry of the Republic of Tajikistan. Intraspecific hybrids were obtained by introducing argali alleles (Ovis ammon) into the gene pool of the Hissar (n = 8), Tajik meat and wool (n = 8) and Pamir fine-fleece (n = 7) sheep breeds. The material for the study consisted in ear notches from hybrid animals with 1/8 argali blood. Polymorphisms at 10 STR loci were studied using ABI3130xl genetic analyzer. Statistical processing of the results was performed using GenAIEx 6.503 program and R diveRsity batch. Hybrid sheep were inferior to maternal forms in allelic diversity and the effective number of alleles: 5.10–5.13 versus 5.51–6.10 and 3.69–3.85 versus 4.49–5.78, respectively. The observed heterozygosity in hybrids with Tajik meat and wool breed was significantly higher than that of mothers, by 13.82%, or 0.119. In hybrids with Hissar and Pamir fine-fleeced breeds, similar changes were tendentious. Hybrid sheep had an excess of heterozygotes (17.0–23.0%), while maternal sheep had their deficiency (1.5–7.8%). In hybrid offspring, argali alleles were found, the frequency of which was insignificant in maternal native Tajik sheep breeds. When analyzing the main coordinates and studying the structure of phylogenetic trees, hybrid forms were located at the junction of the “wild” and “farm” clusters, which corresponds to their origin. The wild-type genetic differentiation in hybrid forms (Fst = 0.199–0.223 and D(N) = 0.921–1.137) was lower than in domestic sheep (Fst = 0.230–0.255 and D(N) = 1.350–2.446). The presence of changes in the gene pool of hybrid forms of sheep that occurred as a result of the introduction of argali alleles was confirmed.
Keywords: microsatellites; hybridization; introduction; domestic sheep; genetic diversity; allele pool.
Author Details: T. E. Deniskova, Cand. Sc. (Biol.), senior research fellow; N. А. Radjabov, Cand. Sc. (Agr.), doctoral student; V. A. Bagirov, D. Sc. (Biol.), corresponding member of the RAS, chief research fellow; N. A. Zinovieva, D. Sc. (Biol.), member of the RAS, director.
For citation: Deniskova TE, Radjabov NА, Bagirov VA, et al. [Effect of the introduction of argali alleles on the gene pool of Tajik native sheep breeds]. Dostizheniya nauki i tekhniki APK. 2020;34(3):41-5. Russian. doi: 10.24411/0235-2451-2020-10308.