M. V. Yakimenko, S. A. Begun, A. I. Sorokina
All-Russian Research Institute of Soybean Breeding, Ignat’evskoe sh., 19, Blagoveshchensk, Amurskaya obl., 675027, Russian Federation
Abstract. The purpose of the studies was to select the optimal carbohydrate for the cultivation and selection of pure Bradyrhizobium japonicum (Jordan, 1982) and Sinorhizobium fredii (Scholla and Elkan, 1984) soybean rhizobia cultures, which assimilate a wide range of carbon nutrition sources. The growth rate of collection strains of soybean rhizobia (91 strains) was studied in vitro in nutrient medium No. 79 with various carbohydrates (maltose, glucose, sucrose, and lactose). On the 7th day of growth, 85% of B. japonicum strains in a nutrient medium without an additional carbon source showed a meager increase in the stroke of a pure culture; 9% of strains showed moderate growth. With the addition of maltose, glucose, sucrose, and mannitol, the growth rate of the bacterial mass of the studied strains increased. The number of B. japonicum strains that showed moderate and rapid growth in pure culture with these components added to the nutrient medium ranged from 38% (sucrose) to 60% (mannitol). The best growth rate of pure B. japonicum cultures was noted in the options with mannitol and glucose. The medium with the addition of lactose for B. japonicum was the least successful: 77% of the studied strains showed meager growth, 15% did not show any growth. At the same time, 75% of S. fredii strains in a similar nutrient medium showed abundant and intensive growth in pure culture. Strains of a fast-growing species hardly grew on a nutrient-free medium without carbohydrate but showed an intensive growth of the bacterial mass with all the tested carbon nutrition sources. The greatest number of S. fredii strains that showed abundant and intensive growth (82%) was observed in the options with the addition of mannitol and sucrose. We identified 4 strains of B. japonicum and 23 strains of S. fredii, which showed good assimilation of all the tested sources of carbon nutrition (mannitol, maltose, glucose, sucrose, lactose).
Keywords: soybean rhizobia; strain; species; B. japonicum; S. fredii; mannitol; maltose; glucose; sucrose; lactose; growth rate.
Author Details: M. V. Yakimenko, Сand. Sc. (Biol.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); S. A. Begun, Сand. Sc. (Biol.), leading research fellow A. I. Sorokina, Сand. Sc. (Vet.), leading research fellow.
For citation: Yakimenko M V, Begun SA, Sorokina AI. [Assessment of the growth rate of Bradyrhizobium japonicum and Sinorhizobium fredii strains bred in the Far Eastern in media with various carbohydrates]. Dostizheniya nauki i tekhniki APK. 2020;34(6):33-7. Russian. doi: 10.24411/0235-2451-2020-10606.