S. I. Lavrentieva, O. N. Tarasova, V. A. Kuznetsova, G. V. Kubankova, D. K. Chernyshuk
All-Russian Research Institute of Soybean Breeding, Ignat’evskoe sh., 19, Blagoveshchensk, Amurskaya obl., 675027, Russian Federation
Abstract. The purpose of the studies was to determine the effect of Septoria glycines Hemmi infection on the biochemical composition and level of low molecular weight metabolites of the antioxidant complex in seeds and seedlings of soybean (Glycine max (L.) Merr.) of Lidia variety. The seeds infected and not infected with Septoria were selected in the Amur Region in 2019. The content of protein, oil, and higher fatty acids (HFA) was determined by near-infrared spectroscopy; the content of isoflavones was determined by high-performance liquid chromatography; the content of ascorbic acid was determined by the titrimetric method; the content of malondialdehyde and carotene was determined by photocolorimeter. Upon infection with Septoria, the content of ascorbic acid in soybean seeds and seedlings increased by 4.55 mg% and 10.51 mg%, respectively; the content of carotene increased by 0.012 and 0.072 mg%, respectively. In uninfected and infected seeds, we detected high concentrations of glycitin (1.4 mg/g and 3.4 mg/g, respectively) and genistin (2.2 mg/g and 3.5 mg/g, respectively) and low concentrations of daidzin (0.3 mg/g and 0.1 mg/g, respectively) and daidzein (0.1 mg/g and 0.2 mg/g, respectively). In infected soybean seedlings, the level of almost all the studied isoflavones (except for daidzin) increased, due to their active participation in the growth and development of the plant. Under the influence of the biotic factor, the content of oleic acid in soybean seeds decreased from 15.1% to 8.4%; the content of linolenic acid increased from 5.6% to 8.6%. The high content of linolenic acid in infected soybean seeds correlated with an increase in the protein content. New information on the metabolism of low molecular weight antioxidants (ascorbic acid, carotene, isoflavones), protein, oil, and high fatty acids can be used in the selection process of soybean varieties resistant to Septoria.
Keywords: soy; Septoria; antioxidants; isoflavones; ascorbic acid; carotene; higher fatty acids.
Author Details: S. I. Lavrentieva, Cand. Sc. (Biol.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); O. N. Tarasova, junior research fellow; V. A. Kuznetsova, senior research fellow; G. V. Kubankova, senior research fellow; D. K. Chernyshuk, research fellow.
For citation: Lavrentieva SI, Tarasova ON, Kuznetsova VA, et al. [Biochemical composition of soybean seeds and seedlings infected with Septoria glycines Hemmi]. Dostizheniya nauki i tekhniki APK. 2020;34(6):38-42. Russian. doi: 10.24411/0235-2451-2020-10607.