L. I. Belyaeva, M. K. Pruzhin, A. V. Ostapenko, V. N. Labuzova, T.I. Sysoeva
Kursk Federal Agricultural Research Center, ul. Karla Marksa, 63, Kursk, 305029, Russian Federation
Abstract. The purpose of the studies was to determine the effectiveness of the glycosidase enzyme preparation Dextrasept 2 in the processing of sugar beet roots infected with mucous bacteriosis. The object of the study was freshly dug and stored roots grown in chernozem soils of the Kursk region and harvested in 2018–2019 and semi-finished sugar beet products. Laboratory experiments were conducted by modelling production processes on the basis of a second-order rotatable plan for two factors at three variation levels with the construction of correlation and regression dependencies and the calculation of a generalized optimization parameter. Bacterial infection of the root cell sap had a negative effect mainly on the technological parameters of semi-finished sugar products, which can be reduced by using glycosidase-active enzymes. The introduction of the complex preparation Dextrasept 2 containing dextranase and levanase enzymes ensured the improvement of technological parameters of semi-finished products, including an increase in the sucrose content in diffusion sap on average by 2.3% to the weight of dry matter and a decrease in the content of high molecular weight compounds by 43.4%; an increase in the purification effect of diffusion sap on average by 3.5%, a decrease in the turbidity of purified sap by 24%, which can provide an increase in the quality and yield of white sugar on average by 0.5%. The use of the preparation Dextrasept 2 at a dose from 4 kg to 8 kg per 1000 tons of sugar beet was effective in the presence of 4–8 pcs of mucous inclusions in the root cell sap. The most technologically acceptable entry points for the Dextrasept 2 preparation, taking into account the degree of bacterial infection of the sugar beet cell sap, were a belt conveyor for feeding washed root crops to the collection tank before beet slicers; a conveyor belt, on which beet chips are transferred to a diffusion unit; collection tank of pulp catcher for diffusion sap; collection tank for diffusion sap.
Keywords: enzyme preparation; glycosidase activity; sugar beet; bacterial infection; polysaccharide; semi-finished product; white sugar; technological parameters.
Author Details: L. I. Belyaeva, Cand. Sc. (Tech.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); M. K. Pruzhin, D. Sc. (Agr.), leading research fellow; A. V. Ostapenko, senior research fellow; V. N. Labuzova, senior research fellow; T.I. Sysoeva, research fellow.
For citation: Belyaeva LI, Pruzhin MK, Ostapenko AV, Labuzova VN, Sysoeva TI [Performance of glycosidase-active enzymes in the production of beet sugar]. Dostizheniya nauki i tekhniki APK. 2020; 34(7):102-8. Russian. doi: 10.24411/0235-2451-2020-10718.