Study of the possibility of using «wild» bacterial strains as possible producers of vitamin B12 (cobalamin)

M. V. Marchenok^1,2, A. B. Podvolotskaya^1,2, E. S. Fishchenko^1,2
1Arnika, ul. Eniseiskaya, 23b, Vladivostok, Primorskii krai, 690039, Russian Federation
²Far Eastern Federal University, ul. Sukhanova, 8, Vladivostok, Primorskii krai, 690091, Russian Federation

Abstract. The purpose of the study was to find new promising microbial producers of vitamin B12 for its synthesis. We screened eight “wild” strains of microorganisms isolated from crop production as possible producers of cobalamins: 1 – Pseudomonas aeruginosa, 1 – Bacillus subtilis, 2 – Bacillus circulans, 3 – Pseudomonas fluorescens, 1 – Pseudomonas denitrificans. At the first stage, they were assessed by the accumulation of biomass under the same cultivation conditions. Strains B. subtilis B1 and P. denitrificans P5 had an advantage in terms of this indicator under aerobic conditions: the yield of dry biomass averaged 11.2 g/L and 12.6 g/L, respectively. At the second stage, the dynamics of bacterial biomass accumulation was studied in terms of optical density, depending on the sources of carbohydrates used (sucrose, glucose, maltose). The results on media with glucose and maltose did not differ statistically: 2.52 relative units (RU) and 2.46 RU for P. denitrificans P5 and 2.50 RU and 2.49 RU for B. subtilis B1. Significantly less biomass accumulated in nutrient media with the addition of sucrose. At the third stage, after fermentation, the concentration of cobalamins in cell extracts was measured using HPLC. B1 strain of B. subtilis produced 0.037 mg/mL of the cyanocobalamin; P5 strain of P. denitrificans produced 0.042 mg/mL. Considering the technological complexity of the B. subtilis cultivation process (alternation of aerobic and anaerobic phases), it is preferable to use the P. denitrificans strain for further research.

Keywords: producer; nutrient medium; cobalamin; vitamin B12; Bacillus subtilis; Pseudomonas denitrificans.

Author Details: M. V. Marchenok, post graduate student, research bioengineer (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); A. B. Podvolotskaya, Cand. Sc. (Med.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E. S. Fishchenko, Cand. Sc. (Tech.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).

For citation: Marchenok MV, Podvolotskaya AB, Fishchenko ES. [Study of the possibility of using «wild» bacterial strains as possible producers of vitamin B12 (cobalamin)]. Dostizheniya nauki i tekhniki APK. 2020;34(7):109-13. Russian. doi: 10.24411/0235-2451-2020-10719.