V.S. Popov, N.V. Vorobieva
Kursk Federal Agrarian Scientific Center, ul. Karla Marksa, 70b, Kursk, 305021, Russian Federation
Abstract. The article presents the results of studies on the cultivation of Bifidobacterium bifidum in a high-carbohydrate nutrient medium based on beet molasses and the scientific and practical substantiation of the possibility of using the culture liquid as a biologically active additive for correcting the microbiocenosis of the gastrointestinal tract in animals. The material for laboratory studies was sugar beet molasses at a concentration of 5–25% (in the control, the concentration was 100%). For its fermentation, we used the probiotic microorganism Bifidobacterium bifidum strain No. 1. The bacterial concentrate was obtained using a daily culture of microorganisms, which was standardized to 1x10E6 CFU/cm3. Experimental studies were conducted on 30 healthy 30-day old suckling piglets (during the period of preparation for weaning) and on 60-day old piglets using the mixed feed on a plant-grain basis. According to the results of testing Bifidobacterium bifidum, the mass fraction of the sum of fermentable sugars ranged from 53.7% to 23.7%; pH ranged from 6.70 to 3.77; the maximum population of Bifidobacterium bifidum in the experiment was noted during the cultivation of microorganisms in a nutrient medium containing 5% of molasses on the second day – 7x10E6 CFU/cm3. The concentration of essential and conditionally nonessential amino acids, as well as organic acids in the experimental sample was significantly higher than in the original molasses. In the microbiocenosis of suckling piglets prevailed B. bifidum and Lactobacillus; their population varied within 10E7–10E9 CFU/g. Weaned piglets aged 60 days showed a decrease in the number of lactobacilli to 10E3 CFU/g, an increase in staphylococci and clostridia to 10E6 CFU/g and 10E7 CFU/g, respectively, a slight increase in other opportunistic microflora. At the same time, the concentration of bifidobacteria remained at the level of 10E9 CFU/g.
Keywords: probiotic microorganisms; metabolites; microbiocenosis; nutrient medium; culture liquid; piglets; intestinal microflora.
Author Details: V. S. Popov, D. Sc. (Vet.), head of laboratory (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N. V. Vorobieva, Cand. Sc. (Vet.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Popov VS, Vorobieva NV. [Scientific and practical substantiation of the possibility of molasses application for the cultivation of Bifidobacterium bifidum]. Dostizheniya nauki i tekhniki APK. 2021;35(1):48-51. Russian. doi: 10.24411/0235-2451-2021-10109.