Anti-immunoglobulin activity of bacteria of the genus Enterococcus isolated from animals

E. E. Kochkina¹, O. L. Kartashova^1,2, M. V. Sycheva^1,2
1Orenburg State Agrarian University, ul. Chelyuskintsev, 18, Orenburg, 460014, Russian Federation
²Institute of Cellular and Intracellular Symbiosis, Ural branch, Russian Academy of Sciences, ul. Pionerskaya, 11, Orenburg, 460000, Russian Federation

Abstract. Conditionally pathogenic microorganisms, striving to survive in a biotope, realize their persistent properties, thanks to which they acquire the ability to withstand the factors of natural resistance of the macroorganism. An important persistence factor that allows microorganisms to resist the bactericidal action of immunoglobulins and to stay inside the macroorganism for a long time is anti-immunoglobulin activity (AIgA). The purpose of the study was to characterize the ability of enterococci isolated from various biotopes of the body of healthy animals and in pathology to inactivate secretory IgA (sIgA). In vitro experiments were conducted on 75 strains of enterococci of different species isolated from the intestinal biotope of healthy productive animals and birds, as well as from animals with infectious and inflammatory diseases; the sIgA-protease activity of microorganisms was determined by Bukharin’s method (2004). AIgA against secretory immunoglobulin A is widespread in the population of faecal and clinical isolates of enterococci. Etiologically significant strains significantly (p was less than 0.01) more often possess AIgA against sIgA than intestinal isolates (61.8% versus 31.7%). The level of the studied persistence factor is associated with the species characteristics of enterococcal cultures. The maximum prevalence and severity of AIgA in the experiment was characteristic of E. faecalis strains. Cultures of enterococci isolated from the intestines of birds were distinguished by a higher prevalence and severity of the trait (50.0% and 21.0%, respectively) than faecal isolates. The results of a comparative analysis of the frequency of occurrence and severity levels of AIgA can be used to differentiate enterococci isolated from the body of animals into etiologically significant strains and representatives of the mutualistic microbiota that opens up prospects for the selection of avirulent production-valuable enterococci cultures.

Keywords: anti-immunoglobulin activity; Enterococcus spp.; persistence factor; sIgA.

Author Details: E. E. Kochkina, post graduate student (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); O. L. Kartashova, D. Sc. (Biol.), prof., head of laboratory; M. V. Sycheva, D. Sc. (Biol.), head of department, senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).

For citation: Kochkina EE, Kartashova OL, Sycheva MV. [Anti-immunoglobulin activity of bacteria of the genus Enterococcus isolated from animals]. Dostizheniya nauki i tekhniki APK. 2021;35(1):43-7. Russian. doi: 10.24411/0235-2451-2021-10108.