N. A. Dengis, A. N. Novikov, N. S. Boganets
Omsk Agrarian Scientific Center, ul. Koroleva, 26, Omsk, 644027, Russian Federation
Abstract. The study aimed to increase the efficiency and reliability of the cultural method for diagnosing tuberculosis using ozone. The work was performed at the Omsk Agricultural Scientific Center. In the experiments, we used biomaterial from cattle reacting to tuberculin purified protein derivative (PPD) from the tuberculosis-free and tuberculosis-infected farms in the Omsk, Tyumen, and Sverdlovsk regions. We conducted a comprehensive study of 68 samples of biomaterial. When sowing on nutrient media, we used a method previously tested on archival cultures. For this, saline solution was saturated with ozone to a concentration of 0.25 mg/L by direct bubbling with an ozone-oxygen mixture on an A-C-GOKSF-5-04-OZON ozone synthesizer. The platings with a standard saline solution served as a control. In parallel with the culture method, the biological material was examined by applying a biological sample to guinea pigs. The virulent and pathogenic properties of cultures grown in an environment with and without ozone were identical, that is, ozone did not affect them. The guinea pigs subcutaneously infected with mycobacterium tuberculosis obtained with the use of ozone developed an inflammatory infiltrate at the injection site on the 5th or 6th day; this infiltrate then turned into an abscess. The regional lymph nodes increased in the period from the 6th to the 10th day reaching the size of a pea by the 30th day. In postmortem examination, the average index of damage to internal organs according to Togunova was 28.1±1.2. A positive biological test using ozonized saline on a model of guinea pigs was consistent with the results of the culture method. The average inoculation rate of tuberculosis mycobacteria from biomaterials from experimentally in vivo infected guinea pigs was significantly higher than in the control – by 3.5 times.
Keywords: Mycobacterium tuberculosis; atypical mycobacteria; growth rate and intensity; ozone; biological test.
Author Details: N. A. Dengis, Cand. Sc. (Biol.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); A. N. Novikov, Cand. Sc. (Vet.), leading research fellow; N. S. Boganets, Cand. Sc. (Vet.), senior research fellow.
For citation: Dengis NA, Novikov AN, Boganets NS. [Features of the indication of mycobacteria using ozone in modern laboratory practice] Dostizheniya nauki i tekhniki APK. 2021;35(5):55-8. Russian. doi: 10.24411/0235-2451-2021-10509.