N. A. Kol’berg, S. L. Tikhonov, N. V. Tikhonova, S. A. Leont’eva
Ural State University of Economics, ul. 8 Marta/Narodnoi Voli, 62/45, Ekaterinburg, 620144, Russian Federation
Abstract. The study assessed the effect of a biologically active additive (BAA) from the bursa of Fabricius of broiler chickens on cytotoxicity and nonspecific immunity of mice against the background of experimental Salmonella infection. For the experiment on cytotoxicity, the additive at concentrations from 0.02 mg/mL to 10.0 mg/mL was introduced into cell cultures of lines L929, J774.1A, HeLaS3, K562, HCT116, and MCF-7 and incubated for 48 hours. To determine the effect of BAA on the nonspecific immunity of mice, we divided white outbred mice (males/females, 20 ± 2 g) into four groups, 30 animals each. Animals from the first group (control) did not receive BAA. Mice from the second experimental group were fed daily for seven days with the additive at a dose of 150 mg/kg; for mice from the third experimental group, the dose was 750 mg/kg; for mice from the fourth experimental group, the dose was 3,750 mg/kg. Thereafter, the animals were inoculated intraperitoneally with the culture of Salmonella enteritidis 92. We established that the additive did not cause a metabolic response and did not change cytotoxic properties and cell viability in cultures L929, J774.1A, HeLaS3, K562, and HCT116. The additive at concentrations of 5 mg/mL and 10 mg/mL had a cytotoxic effect on tumour cells of MCF-7 line, which led to a significant decrease in their viability by 18.5% and 23.8% in comparison with pure cell culture. The BAA from the bursa of broiler chickens activated nonspecific immunity against Salmonella infection, which was expressed in an increase in the number of survivors and postponement of the mean day of death of animals when infected with S. enteritidis at a dose of 5 CFU by 6.3–7.7 times and by 17.8–40.3%, respectively. The most effective BAA doses were 750 mg/kg and 3,750 mg/kg of body weight. In these options, with the introduction of 500 CFU of S. enteritidis, seven and nine animals, respectively, survived, and when fed with BAA at the dose of 150 mg/kg, all animals died.
Keywords: biologically active additive; raw materials of tissue origin; bursa of Fabricius; white outbred mice; cytotoxicity; Salmonella infection; nonspecific immunity.
Author Details: N. A. Kol’berg, Cand. Sc. (Vet.), assoc. prof.; S. L. Tikhonov, D. Sc. (Tech.), prof.; N. V. Tikhonova, D. Sc. (Tech.), prof.; S. A. Leont’eva, post graduate student.
For citation: Kol’berg NA, Tikhonov SL, Tikhonova NV, et al. [Evaluation of the effect of a biologically active additive from the lymphoid tissue of broiler chickens on cytotoxic activity and nonspecific resistance to infections] Dostizheniya nauki i tekhniki APK. 2021;35(7):69-72. Russian. doi: 10.53859/02352451_2021_35_7_69.