N. S. Lyakhovchenko, A. A. Chepurina, I. A. Artemieva, V. Yu. Senchenkov, A. A. Sirotin, I. P. Solyanikova
Belgorod State National Research University, ul. Pobedy, 85, k. 14, Belgorod, 308015, Russian Federation
Abstract. Biologizing agricultural production is an urgent task. At the same time, the replacement of chemical agents to combat harmful organisms with biological agents requires the search for new highly antagonistically active microbial strains. The purpose of the study was to find microorganisms with a pronounced antagonistic effect against phytopathogens and isolate them from the soil into a pure culture, as well as to characterize them based on their phenotypic properties. A Gram-negative spore-forming aerobic rod-shaped bacterium was isolated from a sample of arable chernozem soil using the clumped sowing method. It used D-glucose, sucrose, raffinose, galactose, mannitol, sodium citrate, maltose, lactose, and fructose as a carbon source and formed ammonia during growth in a peptone medium. We did not record any activity of lecithinase, lipase, or catalase. Tests for oxidase and indole formation were negative. The bacterium dissolved inorganic phosphate, slowed the growth of the phytopathogenic mould Alternaria brassicicola F-1864 by two times, and reduced the mean increment, relative to the control, by 20%. Based on the study of the dynamics of mobile phosphorus content in the medium, we assumed that under conditions of a deficiency of this element the isolated strain can dissolve inorganic phosphates and consume them for growth. The isolate was phenotypically similar to representatives of Gardnerella, Streptobacillus, and other genera. Differences in characteristics (for example, formation of ammonia, ability to use some substrates for growth) did not exactly define the taxonomic affiliation of the strain; therefore, further studies of this bacterium are needed.
Keywords: enzymatic activity; soil; agrobiotechnology; phosphatase activity; phytopathology; fungistatic activity; plant protection products; Alternaria brassicicola.
Author Details: N. S. Lyakhovchenko, post graduate student (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); A. A. Chepurina, student; I. A. Artemieva, master’s student; V. Yu. Senchenkov, post graduate student; A. A. Sirotin, Cand. Sc. (Biol.), prof.; I. P. Solyanikova, D. Sc. (Biol.), prof.
For citation: Lyakhovchenko NS, Chepurina AA, Artemieva IA, et al. [Antagonistic and phosphate solubilizing activity of Gram-negative spore-forming soil bacteria]. Dostizheniya nauki i tekhniki APK. 2021;35(8):4-8. Russian. doi: 10.53859/02352451_2021_35_8_4.