D. I. Petrukhina, N. A. Vasil’eva, I. V. Polyakova, V. A. Kharlamov, S. A. Gorbatov, P. N. Tsygvintsev
All-Russian Research Institute of Radiology and Agroecology, Kievskoe sh., 109 km, Obninsk, Kaluzhskaya obl., 249032, Russian Federation
Abstract. The study aimed to evaluate the impact of low-temperature atmospheric pressure argon plasma on Phytophthora spp. to substantiate the development of a methodology for the pre-sowing treatment of potato tubers. An isolate of Phytophthora spp. was provided by Lorch Federal Potato Research Center. The paper considers the following irradiation options: spores sown on a dense nutrient medium (1 min, 3 min, 5 min, and 10 min); mycelium inoculated onto a solid nutrient medium (5 min and 15 min); dry-air mycelium (5 min, 10 min, and 15 min). The plasma was generated by an experimental source designed at the institute. In the control, the tubers were not treated. The distance from the irradiated object to the plasma source was 13 cm; the argon flow rate was 4.5 L/min. By the 10th day of observation, after 1 min of irradiation of the seeded spores of Phytophthora spp. the amount of microorganisms was lower than in the control by 87.2%; after 5 minutes of treatment, it was lower by 98.9%. In the option with 10 min of radiation, the inoculated spores were inactivated. After 5 min of plasma treatment of pieces of fresh mycelium, its diameter on the 15th day of observation was less than in the control by 7.6%. In the option with irradiation for 15 min, the diameter of the mycelium on the 6th day decreased by 12% compared to the control. By the 10th day, plasma irradiation of dry-air mycelium for 10 min and 15 min reduced the number of microorganisms, compared with the control, by 97.3% and 98.6%, respectively. To inactivate the mycelium and spores of Phytophthora spp., which persist on the surface of infected tubers, treatment with low-temperature plasma for 10 minutes can be applied.
Keywords: microwave plasma; plant protection; inactivation; phytopathogens; late blight.
Author Details: D. I. Petrukhina, Cand. Sc. (Biol.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N. A. Vasil’eva, Cand. Sc. (Biol.), senior research fellow; I. V. Polyakova, acting head of the laboratory; V. A. Kharlamov, Cand. Sc. (Biol.), senior research fellow; S. A. Gorbatov, research fellow; P. N. Tsygvintsev, Cand. Sc. (Biol.), acting head of the laboratory.
For citation: Petrukhina DI, Vasil’eva NA, Polyakova IV, et al. [Effect of low-temperature plasma on Phytophthora spp.]. Dostizheniya nauki i tekhniki APK. 2022;36(1):8-12. Russian. doi: 10.53859/02352451_2022_36_1_8.