V. S. Maslennikova1,2, V. P. Tsvetkova1, E. V. Bedareva1,2, G. V. Kalmykova2, I. M. Dubovskii1,2
1Novosibirsk State Agrarian University, ul. Dobrolyubova, 160, Novosibirsk, 630039, Russian Federation
2Siberian Federal Research Center of Agricultural Biotechnology, Russian Academy of Sciences, pos. Krasnoobsk, Novosibirskii r-n, Novosibirskaya obl., 630501, Russian Federation
Abstract. Studied growth- promoting and fungicidal properties of endophytic bacteria Bacillus thuringiensis spp. morrisoni and Bacillus thuringiensis spp. dacota on potatoes. The assessment of the physiological and biochemical parameters of potatoes, as well as the resistance to Rhizoctonia solani under the influence of entomopathogenic strains, was carried out to clarify the processes of pathogenesis of the pathogen and the mechanisms of action of Bacillus thuringiensis bacteria in Western Siberia. The work was carried out in 2020–2021 in the Novosibirsk region. The experimental design included 6 variants in 5 replicates: treatment of tubers with two strains of Bacillus thuringiensis spp. morrisoni and spp. dacota, in comparison with the control and on an artificially infected background of Rhizoctonia solani. Inhibitory activity of Bt spp. morrisoni was 84.0%, Bt spp. dacota – 63.5%. When minitubers were colonized by the fungus Rhizoctonia solani, inhibition of plant growth and development, a decrease in the concentration of chlorophyll a and b by 1.1–1.2 times, as well as a violation of the redox balance, accompanied by the accumulation of malondialdehyde and increase peroxidase activity, were observed. The impact on this background of bacteria B. thuringiensis spp. morrisoni and B. thuringiensis spp. dacota on plants caused an increase in biomass by 1.1 times, the number and length of stems by 1.4 times, the concentration of photosynthetic pigments by 1.3 times, protein and peroxidase activity. At the same time, the concentration of malondialdehyde, the activity of polyphenol oxidase and the content of ascorbic acid in plants did not change under the influence of bacteria.
Keywords: entomopathogenic bacteria; Bacillus thuringiensis spp. morrisoni; Bacillus thuringiensis spp. dacota; Rhizoctonia solani; potato; MDA; peroxidase; photosynthetic pigments; endophyte.
Author Details: V. S. Maslennikova, research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); V. P. Tsvetkova, Cand. Sc. (Agr.), assoc. prof.; E. V. Bedareva, post graduate student; G. V. Kalmykova, Cand. Sc. (Biol.), research fellow; I. M. Dubovskii, D. Sc. (Biol.), prof.
For citation: Maslennikova VS, Tsvetkova VP, Bedareva EV, et al. [Growth-promoting, antioxidant, and fungicidal properties of endophytic bacteria Bacillus thuringiensis on potatoes infected with rhizoctonia root and stem rot]. Dostizheniya nauki i tekhniki APK. 2022;36(7):49-55. Russian. doi: 10.53859/02352451_2022_36_7_49.