L. P. Khlebova1, E. S. Brovko1, O. V. Bychkova1, O. V. Mironenko1, N. V. Barysheva1,2
1Altai State University, prosp. Lenina, 61, Barnaul, 656049, Russian Federation
2Federal Altai Scientific Center of Agrobiotechnologies, Nauchnyi gorodok, 35, Barnaul, 656910, Russian Federation
Abstract. The studies aimed to assess the infection of hop plants. The work was carried out in 2022 in industrial plantations in the Altai Republic. Testing for the presence of 13 types of viruses and 1 viroid was carried out on 5 varieties (Bryansky, Podvyazny, Smolisty, Northern Brewer, Magnum) in the amount of 142 samples using DAS-ELISA and RT-PCR in real time. The total infection of the material was 66.0%, reaching 100% in varieties Bryansky, Podvyazny, Smolisty, Magnum. SLRSV (Strawberry latent ringspot virus), PVY (Potato virus Y), PLRV (Potato leaf roll virus) and PVS (Potato virus S) were most prevalent with an incidence of 40.5, 63.2, 73.5 and 82.4 % respectively. Mosaic viruses PVY and PVS were diagnosed on hops for the first time in the world, Tobacco ringspot virus (TRSV) – for the first time in the Russian Federation. TRSV occurs in 46.7% of plants of the Smolisty variety. Potato virus X (PVX), potato virus A (PVA), and potato spindle tuber viroid (PSTVd) were not detected. Infection by viruses that pose the greatest potential threat to hops – PNRSV (Prunus necrotic ringspot virus) and HpMV (Hop mosaic virus) – amounted to 5.9 and 9.5%, respectively, and in Podvyazny variety it reached 36.4%. The proportion of plants affected by one of the 14 studied pathogens was 8.2%. Simultaneous infection of plants with two viruses was found in all varieties with a frequency of 9.1-66.6%, the maximum incidence was determined in the Bryansky variety. The most common combination of two types of pathogens combined potato viruses – PLRV with PVS or PLRV with PVY. The prevalence of associations of three or more viruses in the pathogenic complex of most genotypes was revealed. Most often, multicomponent mixed infections were diagnosed in varieties Smolisty, Magnum and Podvyazny with an incidence of 66.7-81.8%.
Keywords: hop (Humulus lupulus L.); variety; industrial plantations; viruses; viroid; virus infection; mixed infection.
Author Details: L. P. Khlebova, Cand. Sc. (Biol.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); E. S. Brovko, post graduate student (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); O. V. Bychkova, Cand. Sc. (Agr.), senior research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); O. V. Mironenko, Cand. Sc. (Biol.), head of laboratory (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N. V. Barysheva, Cand. Sc. (Biol.), head of laboratory (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Khlebova LP, Brovko ES, Bychkova OV, et al. [The prevalence of viruses in hop industrial plantations in the foothills of Altai]. Dostizheniya nauki i tekhniki APK. 2022; 36(4):35-9. Russian. doi: 10.53859/02352451_2022_36_12_35.