V.P. Netsvetaev, L.S. Bondarenko, O.V. Akinshina
Belgorod State Research Institute of Agriculture, Oktyabr’skaya str., 58, Belgorod, 308001, Russia
E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
Summary. The chromosomes, which are crucial for the genetic control of isoenzymes of beta-amylase, were determined based on the analysis of F2. This generation was obtained by hybridization of spring wheat variety Pyrotrix with isogenous lines, developed on the basis of variety Novosibirskaya 67. Separation of beta-amylases was performed in Tris-glycine polyacrylamide gel system (pH 8.3). To identify beta-Amy-A1 locus we marked the chromosome 5AL by genetic factors B1b1 (baldness vs. beardedness). To determine locus beta-Amy-D1, the chromosome 4DL was marked by gene Rht 2. Under these conditions, electrophoresis spectrum of beta-amylase of variety Pyrotrix divided into three areas of activity designated in accordance with the increase in the mobility as symbols from A to C and D. The spectrum of beta-amylases of variety Novosibirskaya 67 depleted and occupied an intermediate zone of mobility – B-zone. The dual component A of beta-amylase of variety Pyrotrix is controlled by locus beta-Amy-A1, located at a distance of 13.70 ± 3.37 % recombination to factors B1b1 (baldness vs. beardedness). The most mobile dual component D of this variety showed linkage with Rht 2 locus, responsible for the growth of wheat plants, at the level of 38.89 ± 4.75 % recombination. Genetic analysis of component B, which is under control of two loci, showed the close value of linkage of beta-amylase gene with Rht2 factor, 33.40 ± 10.31 % recombination. Consequently, the component D of variety Pyrotrix is controlled by beta-Amy-D1 locus.
Keywords: beta-amylase isoenzymes, soft wheat, linkage of genes, chromosome control, recombination,variety Pyrotrix.
Author Details: V.P. Netsvetaev, Dr. Sc. (Biol.), Head of Laboratory (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); L.S. Bondarenko, Junior Researcher; O.V. Akinshina, Junior Researcher.
For citation: Netsvetaev V.P., Bondarenko L.S., Akinshina O.V. Genetic analysis of isoenzymes of beta-amylase in soft wheat. Dostizheniya nauki i tekhniki APK. 2015. T. 29. № 4. pp. 17-19 (In Russ)