V.S. Bronnikov1, S.V. Savitskiy2
1All-Russian Research Institute of brucellosis and tuberculosis of animals, Lermontov str., 93, Omsk, 644001, Russia
2Omsk State Agricultural University, Oktyabrskaya str., 92, Omsk, 644122, Russia
E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.
Summary. Brucellosis still remains an unresolved problem in many countries. Long-term programs of sanitation from the disease on the basis of specific prevention and differential diagnostic studies are accepted. The methods in both areas are constantly improved. But due to the fact that the disease is wide spread in the wildlife and is uncontrolled in the poorest countries of world, its potential danger increases. The diagnostics of brucellosis is imperfect because immunological reactions is the same in infected individuals and that, resistant to the infection. We attempted to differentiate ill and vaccinated animals by physical methods of analysis of blood plasma. Investigations were carried out to determine the possibility of application of spectrophotometric (SP) absorption of biopolymer mixture of blood serum for differential analysis in brucellosis. The object was the model of guinea pig, which was officially recognized at brucellosis. We formed 4 groups from animals-analogues weighing 300…350 g for the experiment. The animals from the first group was infected with reference strain of B. abortus 544 subcutaneously at the dose of one billion microbial cells, the second group of guinea pigs were injected subcutaneously with saline solution, the third group was administered by B. abortus 544 at the dose of 100 microbial cells, the fourth group was vaccinated with a live vaccine strain of B. abortus 19. The blood serum in dilution of 1/50 was investigated by spectrophotometer SF-46 in the wavelength range 180…660 nm in 2 months after infection. Spectrophotometric characteristics of serum of vaccinated, infected and intact animals significantly differ. The study of areas in the range of 300…360 and 380…420 nm is the most informative; the difference in absorption can reach 72…83 millivolts. The dose of infection affects the SP-status of the organism even in 2 months after infection. The differences between vaccinated and infected animals make it possible to carry out reliable differential investigations.
Keywords: spectrophotometry, brucellosis, diagnosis, vaccinated, infected.
Author Details: V.S. Bronnikov, Cand. Sc. (Vet.), Senior Researcher (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); S.V. Savitskiy, Cand. Sc. (Vet.), Assoc. Prof.
For citation: Bronnikov V.S., Savitskiy S.V. Spectrophotometric profiles of absorption of blood serum of vaccinated and infected with brucellosis animals. Dostizheniya nauki i tekhniki APK. 2015. T.29. №4. pp. 53-55 (In Russ)