P.K. Arakelyan1, G.V. Raznitsyna1, T.A. Janchenko1, O.O. Manakova1, S.K. Dimov2, A.S. Dimova2, V.I. Vorobyev2
1All-Russian Research institute of brucellosis and tuberculosis of animals, Lermontov str., 93, Omsk, 644001, Russia
2Institute of experimental veterinary of Siberia and the Far East, Krasnoobsk, Novosibisk region, 630501, Russia
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Summary. The purpose of the work was to study the possibility of using R-antigens in the differential postvaccinal diagnostics of brucellosis of cattle immunized with live weak agglutinogenic vaccines. We used the agglutination test (AT), complement fixation test (CFT) with united brucellar S-diagnosticum, CFT with R-antigens, made by the similar technology from B. ovis (natural R-form) and R-forms of B. abortus, as well as immunodiffusion test (IDT) with O-polysaccharide (O-PS) antigen. At vaccination on free from brucellosis farms in most cases titres of CFT with R-antigens exceeded those of CFT with S-antigens with negative IDT with O-PS antigen and negative or questionable AT, or CFT with R-antigen was positive with negative CFT with S-antigen, which confirms the postvaccinal character of reactions. From 1418 samples of blood serum of immunized cattle from 10 satisfactory farms AT (not more than 100 IU) was positive in 11 samples (0.7 %), CFT with S-antigen (not higher than 1:10) – in 100 samples (7.6 %), CFT with R-antigen (1:5…1:20) – in 104 samples (7.8 %), IDT with O-PS antigen was negative in all samples. The situation was reversed in problem herds. In the study of 26 samples of blood serum of immunized cattle from problem farm AT in titres 200 IU and higher was observed in 19 samples (73.4 %), CFT with S-antigen in dilutions of 1:20 and higher – in 26 samples (100 %), CFT with S-antigen in dilutions of 1:20 and higher – in 5 samples (19.2 %), IDT with O-PS-antigen – in 18 samples (69.2 %). The using of the diagnosticum, made from natural R-form of brucella B. ovis, as R-antigen was more effective in differential diagnostics in comparison with R-antigens based on artificial R-forms B. abortus.
Keywords: brucellosis of cattle, live weak agglutinogenic vaccines, differential postvaccinal diagnostics, CFT with R-antigens, B. ovis, R-form of B. abortus.
Author Details: P.K. Arakelyan, Dr. Sc. (Vet.), Leading Researcher (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); G.V. Raznitsyna1, Researcher; T.A. Janchenko, Cand. Sc. (Vet.), Senior Researcher; O.O. Manakova, Junior Researcher; S.K. Dimov, Dr. Sc. (Vet.), Head of Laboratory; A.S. Dimova, Cand. Sc. (Vet.), Senior Research; V.I. Vorobyev, aspirant.
For citation: Arakelyan P.K., Raznitsyna G.V., Janchenko T.A., Manakova O.O., Dimov S.K., Dimova A.S., Vorobyev V.I. Role of R-antigens in postvaccinal differential diagnostics of brucellosis of cattle immunized with live weak agglutinogenic vaccines. Dostizheniya nauki i tekhniki APK. 2015. T.29. №4. pp. 63-66 (In Russ)