O.Y. Antonova, S.E. Dunaeva, Y.V. Ukhatova, N.Y. Kamylina, N.A. Dolganova, O.V. Lisicyna, T.A. Gavrilenko
Federal Research Center the N. I. Vavilov All-Russion Institute of Plant Genetic Resources (VIR), Bolshaja Morskaja str., 42-44, Saint-Petersburg, 190000, Russia
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Summary. The effect of different antiviral therapy methods on the efficiency of in vitro eradication of raspberry bushy dwarf virus (RBDV) was studied. Raspberry plants of 41 selection varieties from VIR collection was tested for RBDV presence by ELISA. Every variety was presented by field plant and its in vitro analogue. RBDV was detected in 17 varieties from field collection (41.5 %), for 8 of them RBDV was also detected in in vitro plants. We selected microplants of 7 varieties for improvement: Dormanred, Ottawa, Balzam, Vysokaya, Novokitaevskaya, Samarskaya Plotnaya, Sputnitsa. The treatment was carried out using four strategies: chemotherapy on a media with ribavirin (30 mg/L) or with RNase (0.01 %) and a complex therapy which combined the chemotherapy by the same agents and the heat treatment (35deg C). The inhibition of growth and development of microplants of all varieties was observed on the media with ribavirin in compare with that on the media with RNase. The heat treatment also led to inhibition of plants and reduction in their viability. The combination of thermo-therapy with treatment by ribavirin caused the loss almost of half of microplants. Varieties Dormanred, Novokitaevskaya and Samarskaya Plotnaya were the most heat-resistant. Survived clones (from 2 to 22 for each variety, 49 clones in total) were re-tested by ELISA. In the most cases RBDV-free plants were obtained by the complex therapy. The use of ribavirin showed the better results in comparison with RNase (60 % and 25 % of eradicated clones respectively). Chemotherapy without heat treatment was not sufficiently effective; only one eradicated plant was obtained by this method.
Keywords: RBDV, raspberry, thermotherapy, chemotherapy.
Author Details: O.Y. Antonova, Cand. Sc. (Biol.), Leading Researcher (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.), S.E. Dunaeva, Cand. Sc. (Biol.), Senior Researcher; Y.V. Ukhatova, Junior Researcher; N.Y. Kamylina, Junior Researcher; N.A. Dolganova, Reseacher; O.V. Lisicyna, Post-graduate Student; T.A. Gavrilenko, Dr. Sc. (Biol.), Head of Division.
For citation: Antonova O.Y., Dunaeva S.E., Ukhatova Y.V., Kamylina N.Y., Dolganova N.A., Lisicyna O.V., Gavrilenko T.A. In vitro improvement of raspberry varieties from raspberry bushy dwarf virus (RBDV) using complex therapy method. Dostizheniya nauki i tekhniki APK. 2015. V.29. №7. pp. 61-64 (In Russ)