1V.G. Oschepkov, 2N.N. Koshcheev
1Omsk Institute (branch) of the Russian Economic University by G.V. Plekhanov, ul. 10 Let Oktyabrya, 195, corp. 18, Omsk, 644009, Russian Federation
2All-Russian research institute of brucellosis and tuberculosis of animals, ul. Lermontovа, 93, Omsk, 644001, Russian Federation
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Summary. In recent years the problem aggravated of the differentiation of specific and unspecific (para- and pseudoallergic) skin reactions to the diagnostic PPD-tuberculin. There are used several methods (intravenous, simultaneous, palpebral tests, etc.) for differentiate the etiology of these reactions. However, the used methods are rather labor-intensive and do not yielded unambiguous, reliable results in all cases. We studied the possibility to use the intradermal test with recombinant allergens for the differential diagnosis of tuberculosis in cattle. This allergen is made according to fundamentally different modern genetic-engineering technology (the patent of the Russian Federation 2360926). Initially in two experiments with 26 guinea pigs it was revealed, that animals (100%), inoculated with antitubercular BCG vaccine, as well as animals (60%), infected with different types of atypical non-tuberculous mycobacteria (M. xenopi, M. intractllulare, M. scrofulaceum, M. smegmatis), can react to the PPD-tuberculin. And these animals do not react on the new test allergen. In the industrial experiments with cattle from 12 different farms from three region of Russia (Omsk, Tyumen regions, the Republic of Tatarstan), which are safe in tuberculosis, it was confirmed, that the new recombinant allergen (NRA) significantly exceeds PPD-tuberculin on the specificity. 22913 animals were studied by the intradermal test with PPD-tuberculin, and 416 animals (1.7 %) reacted on this allergen. At that no animals reacted to the NRA. We tested three doses on cattle (0.2, 1170-180 and 85-90 microgram/head), and the best results were provided by the dose of 85-90 microgram/head, which was immunologically more specific more economical. In cattle from farms, safe in tuberculosis, the appearing skin allergic reactions to PPD-tuberculin can be reliably differentiated by the new recombinant allergen, using it also in the intradermal test at the dose of 85-90 microgram/head and taking into account, that the reaction appears in 48- 72 hours.
Keywords: bovine tuberculosis, non-specific allergic reactions, differential diagnosis.
Author Details: V.G. Oschepkov, D. Sc. (Vet.), prof., N.N. Koshcheev, Cand. Sc. (Vet.), senior researcher fellow (E-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.)
For citation: Oschepkov V.G., Koshcheev N.N. New Promising Allergen (NRA) for the Diagnosis of Tuberculosis in Animals. Dostizheniya nauki i tekhniki APK. 2015. V.29. No10. pp. 91-94 (In Russ)