R.R. Vаfin1, S.V. Tyulkin2,3, L.R. Zagidullin3, A.V. Muratova3, T.M. Ahmetov3, F.F. Zinnatova1, Yu.R. Yulmetieva1, Sh.K. Shakirov1, M.Sh. Tagirov1, R.Kh. Ravilov3
1Tatarian Agricultural Research Institute, ul. Orenburgskii trakt, 48, Kazan’, 420059, Russian Federation
2Tatar Interregional Veterinary Laboratory, ul. Rodiny, Kazan’, 420087, Russian Federation
3N.E. Bauman Kazan State Academy of Veterinary Medicine, ul. Sibirskii trakt, 35, Kazan’, 420029, Russian Federation
Abstract. The study was carried out in 2016. The aim of the investigation was a comparative analysis of the frequency distribution of alleles and genotypes of growth hormone-releasing hormone (GHRH) and insulin-like growth factor (IGF-1) genes in bulls in the Republic of Tatarstan. Blood samples were taken from 70 purebred and hybrid bulls of Holstein breed from GUP GPP “Elita”, Vysokaya Gora district, the Republic of Tatarstan. Genomic DNA was extracted from these samples to carry out molecular genetic studies. The corresponding pairs of oligonucleotide primers (GHRHF and GHRHR, IGF-1F and IGF-1R) were used with matched amplification regimes for PCR. For RFLP analysis the restriction endonucleases HaeIII and BstSNI were used, providing genotyping based on the interpretation of the HaeIII-PCR-RFLP profiles of genotypes AB (242/194/55/48 bp), BB (194/55/48 bp) and AA (242/55 bp) of the GHRH gene, as well as BstSNI-PCR-RFLP profiles of genotypes AB (249/223/26 bp), BB (249 bp) and AA (223/26 bp) of the IGF-1 gene. Two alleles (A and B) and three genotypes (AA, AB and BB) of GHRH and IGF-1 genes are revealed in the studied population. Frequency of occurrence of A and B alleles of GHRH and IGF-1 genes was 0.19 and 0.81, 0.48 and 0.52, respectively. The frequency of occurrence of genotypes AA, AB, and BB was 2.9 %, 31.4 % and 65.7 % for GHRH gene; 25.7 %, 44.3 % and 30.0 % for IGF-1 gene, respectively. The occurrence of genotypes BB and AB, allele B of GHRH and IGF-1 genes at purebred and hybrid bulls of Holstein breed was higher than the occurrence of the genotype AA and allele A of the corresponding genes. Based on the Hardy-Weinberg law, we can conclude that the population of breeding bulls by the ratio of the genotypes of the genes GHRH and IGF-1 is in a state of equilibrium.
Keywords: bull, gene, GHRH, IGF-1, polymorphism, PCR-RFLP.
Author Details: R.R. Vafin, D.Sc. (Biol.), prof. of the RAS, chief research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); S.V. Tyulkin, Cand. Sc. (Agr.), head of divison, applicant; L.R. Zagidullin, Cand. Sc. (Biol.), head of department; A.V. Muratova, applicant; T.M. Ahmetov, D. Sc. (Biol.), prof.; F.F. Zinnatova, Cand. Sc. (Biol.), deputy director; Yu.R. Yulmetieva, Cand. Sc. (Biol.), head of laboratory; Sh.K. Shakirov, D. Sc. (Agr.), prof., head of center, M.Sh. Tagirov, D. Sc. (Agr.), academician of TAS, director; R.Kh. Ravilov, D. Sc. (Vet.), prof., head of department, rector.
For citation: Vаfin R.R., Tyulkin S.V., Zagidullin L.R., Muratova A.V., Ahmetov T.M., Zinnatova F.F., Yulmetieva Yu.R., Shakirov Sh.K., Tagirov M.Sh., Ravilov R.Kh. Polymorphism of Genes of Growth Hormone-Releasing Hormone and Insulin-Like Growth Factor in Bulls in the Republic of Tatarstan. Dostizheniya nauki i tekhniki APK. 2017. V. 31. No. 4. Pp. 75-78 (in Russ.).