E.V. Rogozina1, V.A. Biryukova2, E.A. Simakov2, V.A. Zharova2, N.A. Chalaya1, M.A. Kuznetsova3, A.N. Rogozhin3, M.P. Beketova4, O.A. Fadina4, E.E. Khavkin4
1Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), ul. B. Morskaya, 42-44, Sankt- Petersburg, 190000, Russian Federation
2A.G. Lorkh All-Russian Research Institute of Potato Farming, ul. Lorkha, 23, pos. Kraskovo, Lyuberetskii r-n, Moskovskaya obl., 140051, Russian Federation
3All-Russian Research Institute of Phytopathology, ul. Institute, vl. 5, r.p. Bol’shie Vyazemy, Odintsovskii r-n, Moskovskaya obl.,143050, Russian Federation
4All-Russian Research Institute of Agricultural Biotechnology, ul. Timiryazevskaya, 42, Moskva, 127550, Russian Federation
Abstract. The objective of the study was to clarify the perspective of usage of 60 interspecific hybrid clones as parents for potato breeding program focusing on resistance to pest and diseases, including marker-assisted breeding approach. Hybrid clones were assessed in 2014–2016 on the complex of important traits (agronomic and physiological features and product quality) and after artificial inoculation with late blight, potato virus Y, golden nematode of Ro1 pathotype and potato wart. We carried out a DNA-analysis of hybrid clones to the presence of SCAR-markers of genes of resistance to late blight (R1, R2, R3a, R3b, RB/Rpi-blb1 = Rpi-sto1, Rpi-vnt1.3), golden nematode (H1, Gro1-4) and potato virus X (Rx1), or STS-markers of genes of resistance to white potato cyst nematode (Gpa2-2), Y-potato virus (Ry sto, Ry adg) and to potato wart (Sen1). We recognized hybrid clones encompassing numerous economically valuable traits as well as resistance to several pathogens: clone 134-6-2006 possesses resistance to late blight and two nematodes (pathotype Ro1 Globodera rostochiensis and Pa2 G. pallida), clones 117-2, 39-1-2005 and 24-1 possess resistance to late blight and pathotype Ro1 G. rostochiensis, clones 194-4t, 171-3 and 38 KBA possess resistance to late blight and potato virus Y, clones 135-3-2005, 8-1-2004 and 8-5-2004 possess resistance to nematode Ro1 G. rostochiensis and potato virus Y, both clones 94-5 and 99-10-1 possess resistance to potato virus X and potato virus Y. Some hybrid clones: 99-6-6, 135-1-2006, 97-155-1, 14/08-06 and 38КВА selected among progenies from crosses between diverse wild and cultivated potato species were identified as outstanding due to their combinations of R-genes controlling resistance to various strains of Phytophthora infestans Mont. de Bary. DNA markers for six R-genes: R3a, R3b, Rpi-vnt1.3, Rx1, H1 and Gpa 2, were detected in 134-6-2006 genotype (derivative from S. alandiae); DNA markers for four R-genes: H1, Sen1, Rx1 and Ry sto were detected in 94-5 genotype (with S. chacoense in pedigree).
Keywords: Solanum; Petota; Phytophthora infestans; Globodera rostochiensis; Synchytrium endobioticum; potato Y virus; interspecific hybridization; introgression breeding; DNA markers for R genes.
Author Details: E.V. Rogozina, D. Sc. (Biol.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); V.A. Biryukova, Cand. Sc. (Biol.), head of laboratory; E.A. Simakov, D. Sc. (Agr.), head of breeding center; V.A. Zharova, Cand. Sc. (Agr.), research fellow; N.A. Chalaya, Cand. Sc. (Agr.), junior research fellow; M.A. Kuznetsova, Cand. Sc. (Biol.), head of division; A.N. Rogozhin, Cand. Sc. (Agr.), senior research fellow; M.P. Beketova, Cand. Sc. (Biol.), senior research fellow; O.A. Fadina, Cand. Sc. (Biol.), senior research fellow; E.E. Khavkin, D. Sc. (Biol.), head of laboratory.
For citation: Rogozina E.V., Biryukova V.A., Simakov E.A., Zharova V.A., Chalaya N.A., Kuznetsova M.A., Rogozhin A.N., Beketova M.P., Fadina O.A., Khavkin E.E. Interspecific Hybrids as Parental Lines in Anticipatory Breeding for Potato Resistant to Disease and Pests. Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 1. Pp. 26-31 (in Russ.).