G.F. Hadieva1, М.Т. Lutfullin1, Y.А. Akosah1, А.V. Malova1, N.К. Моchalova1, S.G. Vologin2, Z. Stasevski2, А.М. Mardanova1
1Kazan Federal University, Institute of Fundamental Medicine and Biology, ul. Kremlevskaya, 18, Kazan’, 420008, Russian Federation
2Tatarian Agricultural Research Institute – subdivision of Federal Research Center «Kazan Scientific Center of the Russian Academy of Sciences», ul. Orenburgskii trakt, 48, Kazan’, 420059, Russian Federation
Abstract. We studied the species composition and pathogenic properties of micromycetes of the genus Fusarium isolated from dry-rotted and latent-infected tubers of potato ‘Zhukovsky Ranny’, grown on the territory of the Republic of Tatarstan. Weather conditions during the period of research (2016) were extremely arid with a hydrothermal coefficient of 0.43; the soil was gray forest, heavy loamy. Potato plants were grown on sites of a four-field crop rotation after potato, spring wheat, pea, and fallow. We planted five replications in ten tubers. After harvesting, after 45 days of storage of the tubers at room temperature, 10 samples were taken with dry rot; among healthy specimens, 20 samples for the analysis of latent infection were taken from the visual examination. Micromycetes were cultured on Czapek's medium at 28 C. The generic affiliation of the isolates was established by morphological features using light microscopy, specific affiliation was determined by the ITS-region of the 5.8S rRNA genes, isolated during sequencing, using the NCBI database. A total of 39 Fusarium isolates were isolated. Virulent properties were observed in 17 of 28 isolates (60%) from dry rot affected tubers, and 5 of 11 isolates (45%) in samples with a latent infection. Nine isolates with dry rot and four isolates with latent infection were selected from the tubers, grown after potato. For bare fallow these numbers were 5 and 1, for spring wheat and pea – seven isolates, no latent infection was detected. In the tubers affected by dry rot, we identified 5 isolates of F. oxysporum, 1 isolate of F. sambucinum, 1 isolate of F. sporotrichioides. In tubers with latent infection, we detected 2 isolates of F. oxysporum, 2 isolates of F. solani, in 1 specimen the species identity could not be identified.
Keywords: potato; fusariosis; latent infection; phytopathogenic micromycetes.
Acknowledgements. The work was carried out within the framework of the state program to increase the competitiveness of Kazan Federal University among the world's leading scientific and educational centers, the state task of the Federal Agency for Scientific Organizations of Russia AAAA-A18-118030500073-4, and with the financial support of the RFBR and the Government of the Republic of Tatarstan in the framework of the scientific project No. 17-44-160481.
Author Details: G.F. Hadieva, master’s student (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); М.Т. Lutfullin, engineer; Y.А. Akosah, post graduate student; А.V. Malova, bachelor; N.К. Моchalova, engineer; S.G. Vologin, Cand. Sc. (Biol.), leading research fellow; Z. Stasevski, Cand. Sc. (Biol.), head of division; А.М. Mardanova, Cand. Sc. (Biol.), assoc. prof.
For citation: Hadieva G.F., Lutfullin М.Т., Akosah Y.А., Malova А.V., Моchalova N.К., Vologin S.G., Staszewski Z., Mardanova А.М. Analysis of Fusarium micromycetes, isolated from infected potato tubers grown in the Republic of Tatarstan. Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 3. Pp. 34-39 (in Russ.). DOI: 10.24411/0235-2451-2018-10307.