V. M. Nikolaev1, L. P. Koryakina2, S. A. Fedorova3
1Yakutsk Scientific Center of Complex Medical Sciences, Sergelyakhskoe sh. 4, Yakutsk, 677010, Russian Federation
2M. K. Ammosov North Eastern Federal University, st. Belinskogo, 58, Yakutsk, 677000, Russian Federation
3Yakutsk State Agricultural Academy, st. Krasil’nikova, 15, Yakutsk, 677007, Russian Federation
Abstract. The authors determined the influence of low temperatures (-10 ± 2) C on the processes of lipid peroxidation in tissues of viscera (liver, kidneys, lungs, heart) of rats. An experimental and control group consisted of eight Wistar rats. Animals from the experimental group were exposed to the cold temperature for 14 days. In cold, the temperature of paws, measured by an electric thermometer with a needle sensor, decreased by 6.8 C to (+20.5 ± 0.5) C and of the tail – by 3.7 C to (+21.4 ± 0.7) C, in comparison with the control. It indicates a violation of microcirculation and occurrence of hypoxia in the limbs and, as a result, intensification of peroxide processes. At the end of the experiment, 100 mg of liver, kidney, lung and heart tissue were sampled, which were examined using SPECORD 40 spectrophotometer. In the experimental animals, the concentration of malondialdehyde, the end product of lipid peroxidation, was significantly lower than control values: 1.6 times in the liver tissues (6.64 nmol/g), 1.7 times in kidneys (9.74 nmol/g), 3.0 times in lungs (5.02 nmol/g). The level of diene conjugates, primary products of lipid peroxidation, was reliably higher, in comparison with the intact group, in liver tissues – 1.7 times (3.79 micromol/L), kidneys – 5.6 times (5.55 micromol/L), lungs – 1.4 times (7.10 micromol/L), myocardium – 1.7 times (4.51 micromol/L). The total content of low-molecular antioxidants was reliably higher, in comparison with the control group, in the liver tissues – 1.8 times (29.78 mg-eq/g of quercetine), kidneys – 2.0 times (76.57 mg-eq/g of quercetine), lungs – 22.0 times (44.18 mg-eq/g of quercetine). The catalase activity was significantly lower than the control values: in the liver tissue – 1.4 times (15.14 microkat/g), in kidneys – 1.7 times (10.45 microkat/g), in lung – 1.7 times (9.89 microkat/g), in myocardium – 1.5 times (8.56 microkat/g).
Keywords: low-temperature effect; lipid peroxidation; free radical lipid oxidation; lipoperoxidation; active oxygen species; malondialdehyde; diene conjugates; experimental animals; spectrophotometric methods; electrothermometer with a needle sensor; adaptation.
Author Details: V. M. Nikolaev, Cand. Sc. (Biol.), senior research fellow (е-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); L. P. Koryakina, Cand. Sc. (Vet.), head of department; S. A. Fedorova, D. Sc. (Biol.), head of laboratory.
For citation: Nikolaev V. M., Koryakina L. P., Fedorova S. A. Influence of Low Temperatures on Lipid Peroxidation in Tissues of Experimental Animals. Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 4. Pp. 62-64 (in Russ.). DOI: 10.24411/0235-2451-2018-10415.