O. M. Soboleva
1Kemerovo State Agricultural Institute, ul. Markovtseva, 5, Kemerovo, 650056, Russian Federation
2Kemerovo State Medical University, ul. Voroshilova, 22 а, Kemerovo, 650056, Russian Federation
Abstract. Many microorganisms on the grain surface influence negatively its quality and safety; this affects the health of consumers and also leads to economic losses of manufactures and processers of grain. Electrophysical methods of grain treatment, in particular, processing by the superhigh frequency (SHF) electromagnetic field, can serve as an alternative to the classical methods of reducing microbial contamination (physical, chemical and thermal means). The goal of the research was to study the dynamics of the microorganism population on the surface of winter rye and spring barley grain after SHF-treatment. To create an artificial contamination of the grain surface, the biomass of micromycetes (genera of Fusarium, Aspergillus and Penicillium) was grown on Saburo medium. Bacillus subtilis were grown on nutrient medium for determination of mesophilic aerobic and facultative anaerobic microorganisms. After inoculation, the grain was dried and treated by SHF-field with the magnetron frequency of 2.45 GHz, power of 700 W, and exposure of 0, 5, 15, 45 seconds. The total contamination was 4.3*10E6 CFU/g for rye grain and 4.9*10E6 CFU/g for barley grain. SHF-treatment during 5 seconds significantly increased the number of Bacillus subtilis: up to 7.1*10E8 CFU/g for rye grain (165 times) and up to 6.1*10E7 CFU/g for barley grain (12.7 times). The increase in the exposure to 15 seconds led to a significant decrease in the number of almost all studied groups of microorganisms relative to the control values, on average by 98.81% for rye grain and by 86.28% for barley grain. The maximum sterilizing effect was observed for SHF-treatment with the exposure of 45 seconds – 99.93–99.97% of microorganisms died. The survival rate of microorganisms on the surface of barley grain was higher compared to rye grain; however, at the maximum exposure (45 seconds) it was practically equal: 9.3*10E2 CFU/g for rye and 5.0*10E2 CFU/g for barley.
Keywords: electromagnetic field; SHF; microflora; cereals; winter rye; spring barley; microbial survival rate.
Author Details: O. M. Soboleva, Cand. Sc. (Biol.), assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Soboleva O. M. Dynamics of Microorganism Population on the Surface of Rye and Barley Grains after Electromagnetic Treatment. Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 9. Pp. 21–23 (in Russ.). DOI: 10.24411/0235-2451-2018-10905.