V. G. Kostenko, G. I. Podzigun, V. A. Kovalyonok
All-Russian Research Institute of Starch Products – the branch of the V. M. Gorbatov Federal Science Center of Food Systems of the RAS, ul. Nekrasova, 11, pos. Kraskovo, Lyuberetskii r-n, Moskovskaya obl., 140051, Russian Federation
Abstract. The content of amylose in starch is one of the characteristics of its quality. Basically, it is a species characteristic of the original starch-containing raw material and starch extracted from it. Physical and chemical properties of starch and directions for its use depend on it. Breeders need methods for the determination of amylose in different types of raw materials. But before developing a method for determining amylose on spectrophotometric instruments, it is necessary to investigate its optical properties. Since the optical properties of the components of different types of starch differ, the technique developed for the determination of amylose in maize starch is not suitable for its determination in triticale starch. The purpose of the research was to study the optical properties of starch and iodine polysaccharides from triticale starch and to establish the similarities and differences with the iodine polysaccharides of other types of starch. A significant effect on the optical density of solutions of triticale iodine polysaccharides is exerted by the concentration of iodine and potassium iodide with a KI: I2 ratio from 3 to 15. The three-factor experiment was carried out according to the method of Latin squares. With a content of 0.05–0.06 mg/cm3, iodine had a minimal effect on the optical density of the triticale iodine polysaccharide solution. With an increase in its concentration, the optical density increased in a nonlinear correlation. Potassium iodide affected the optical density to a greater degree than iodine. With a molar ratio of KI: I2 equal to 15, the optical density was 2 times higher than at a molar ratio equal to 5. Therefore, in the test solution, the iodine concentration should be 0.06 mg/cm3 with a molar ratio of KI: I2 equal to 5.
Keywords: triticale starch; iodine amylose; iodine amylopectin; iodine polysaccharide; compounds of inclusions; optical density; wavelength; spectrophotometry.
Author Details: V. G. Kostenko, Cand. Sc. (Tech.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); G. I. Podzigun, Cand. Sc. (Chem.), leading research fellow; V. A. Kovalyonok, D. Sc. (Tech.), leading research fellow.
For citation: Kostenko V. G., Podzigun G. I., Kovalyonok V. A. Optical Properties of Iodine Polysaccharides of Triticale Starch. Dostizheniya nauki i tekhniki APK. 2018. Vol. 32. No. 11. Pp. 82–84 (in Russ.). DOI: 10.24411/0235-2451-2018-11122.