N. V. Proletova
Federal Scientific Center of Bast-Fiber Crops Breeding, ul. Lunacharskogo, 35, Torzhok, Tverskaya obl., 172002, Russian Federation
Abstract. A series of studies was conducted to develop a selective in vitro system for obtaining flax genotypes resistant to anthracnose. In the medium, where the studied strains of the Colletotrichum lini fungus were cultivated, such amino acids as alanine, glycine, asparagine, cysteine, aspartic acid, glutamic acid, arginine and threonine were detected in 7–50 days. An assumption was made that the presence of asparagine, alanine, glycine, aspartic and glutamic acids in culture filtrates (CF) could stimulate the morphogenetic activity of flax cells under selective in vitro conditions and appropriate CF concentrations. Differentiation of flax genotypes in terms of their resistance to anthracnose includes cultivation of donor plants of immature embryos (IE); aseptic isolation of IE; cultivation of IE using a modified Sh-2 medium containing a CF consisting of a mixture of strains; visual assessment of the formed callus; and differentiation of flax genotypes in terms of their resistance to anthracnose. The amount of the morphogenic callus formed in the first and second passages allows an appropriate differentiation of flax species in terms of the resistance. As a result of in vitro selection, 21 stable culture lines were obtained. When these forms were crossed with susceptible varieties, a recessive inheritance of the anthracnose resistance trait occurred in subsequent generations. As a result, the type of inheritance is difficult to determine. Two hybrid forms were selected (NE-38 x Lenok and NE-38 x Rosinka), which retained the pathogen resistance at the level of 62.5–66.7% in the first and subsequent generations.
Keywords: flax; anthracnose; resistance; selective agent; culture filtrate; amino acids; immature embryo; callus.
Author Details: N. V. Proletova, Cand. Sc. (Biol.), leading research fellow (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.).
For citation: Proletova N. V. Use of Biotechnological Methods for the Development of New Flax Genotypes Resistant to Anthracnose. Dostizheniya nauki i tekhniki APK. 2019. Vol. 33. No. 8. Pp. 24–28 (in Russ.). DOI: 10.24411/0235-2451-2019-10805.