S. I. Lavrent’yeva1,2, N. V. Martinenko1, D. K. Chernyshuk1, L. E. Ivachenko1,2
1All-Russian Scientific Research Institute of Soybean, Ignatevsky highway, 19, Blagoveshchensk, Amurskaya obl., 675027, Russian Federation
2Blagoveschensk State Pedagogical University, Lenin street, 104, Blagoveshchensk, Amurskaya obl., 675000, Russian Federation
Abstract. The purpose of the studies was to analyze the response of seeds and seedlings of Lidia soybean variety (Glycine max (L.) Merr.) to stress caused by the fungal infection Septoria glycines Hemmi (S. glycines) to expand the range of protein markers that are necessary for breeding new varieties resistant to S. glycines. Soybean seeds were grown in the Amur region in 2019 on meadow chernozem-like soil. The seeds infected with S. glycines were selected visually by morphological characteristics. The specific activity of hydrolytic enzymes (ribonuclease, acid phosphatase, esterase, and amylase complexes) and the concentration of malondialdehyde were determined spectrophotometrically in soybean seeds and their ten-day-old seedlings infected and uninfected (the control) with septoriosis. Multiple forms of the studied hydrolases were detected by electrophoresis in 7.5% polyacrylamide gel. The data were processed mathematically using the Statistica 10 software. In soybean seedlings infected with S. glycines, we detected an increase in the concentration of malondialdehyde by 17% relative to the control that indicated oxidative stress. We also registered a significant increase in the specific activity of hydrolases of infected soybean seeds, in comparison with the control. The specific activity of ribonuclease increased by 40%; the activity of amylase complex increased by 16%, the activity of esterase complex raised by 33%. The specific activity of the amylase complex in the affected soybean seedlings, in comparison with the control, increased by 7%. The number and electrophoretic mobility of multiple forms of ribonucleases, esterases, and amylases changed in response to the action of the pathogen. The activity of acid phosphatases under conditions of infection remained stable. Ribonuclease is characterized by an increased polymorphism and selective neutral behaviour (in relation to S. glycines in the soybean variety Lydia). This opens up possibilities for its use as a protein marker.
Keywords: soybean (Glycine max); Septoria glycines; oxidative stress; hydrolases; specific activity; multiple forms.
Author Details: S. I. Lavrent’yeva, Сand. Sc. (Biol.), leading research fellow, assoc. prof. (e-mail: Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N. V. Martynenko, junior research fellow; D. K. Chernyshuk, research fellow; L. E. Ivachenko, D. Sc. (Biol.), leading research fellow, prof.
For citation: Lavrent’yeva SI, Martinenko NV, Chernyshuk DK, et al. [Influence of Septoria glycines Hemmi on the activity of hydrolytic enzymes of soybean ‘Lidia’]. Dostizheniya nauki i tekhniki APK. 2020;34(8):33-8. Russian. doi: 10.24411/0235-2451-2020-10805.