S. S. Babanina, N. A. Yegorova, I. V. Stavtseva, I. V. Belova
Crimea Research Agricultural Institute, ul. Kievskaya, 150, Simferopol’, Respublika Krym, 295453, Russian Federation
Abstract. The work aimed to study the effect of the duration of micropropagation in vitro on the characteristics of ex vitro adaptation of microplants Lavandula angustifolia Mill. The material for the study was lavender microplants of the Sineva variety (propagated in vitro during 4, 5, 6, 7, and 8 subculturing) at the stage of ex vitro adaptation. For adaptation, rooted microplants were transferred to a mixture of peat and perlite (1:1) and grown under illumination of 2-3 kLx, photoperiod duration of 16 h, temperature of 24 ± 2C, air humidity of 70-90%, watering was carried out with Knop’s solution. On the 1st, 14th, 30th, 45th and 60th days of adaptation, the number and length of the main and lateral shoots, the mass of the aerial parts of microplants and roots, the amount of photosynthetic pigments in the leaves were analysed, and the functional state of the microplants was also assessed. During adaptation, the growth of the main shoot, the appearance of additional shoots, an increase in the mass of the root system and the aerial part of the plant were observed. The mass of the whole plant for 45 days of adaptation increased by 5 times. Significant differences between the values of these indicators depending on the number of subculturing were not revealed, except for their decrease in microplants obtained after 6 subculturing, with further recovery in the 7th-8th. The number of subcultivations also did not affect the photosynthetic activity of microplants during ex vitro adaptation, and the coefficient of photosynthetic activity and the viability index in plants on 30-45 day of adaptation were 1.1-2.6 times greater than at the stage of rooting in vitro. During 45 days of ex vitro adaptation, the content of chlorophyll a, b and carotenoids increased by 1.2-1.5 times. The results of the study indicate the successful adaptation of microplants to ex vitro conditions and the restoration of their physiological activity on days 30-45.
Keywords: spicate lavender (Lavandula angustifolia Mill.); in vitro micropropagation; number of subcultures; ex vitro adaptation; viability index; photosynthetic activity coefficient; photosynthetic pigments.
Author Details: S. S. Babanina, Cand. Sc. (Agr.), senior research fellow (e-mail: svetlana. Этот адрес электронной почты защищён от спам-ботов. У вас должен быть включен JavaScript для просмотра.); N. A. Yegorova, D. Sc. (Biol.), head of laboratory; I. V. Stavtseva, Cand. Sc. (Biol.), senior research fellow; I. V. Belova, Cand. Sc. (Agr.), research fellow.
For citation: Babanina SS, Yegorova NA, Stavtseva IV, et al. [Influence of clonal micropropagation duration on ex vitro adaptation of microplants Lavandula angustifolia Mill.]. Dostizheniya nauki i tekhniki APK. 2022;36(7):36-42. Russian. doi: 10.53859/02352451_2022_36_7_36.